微量荧光法体外测定恶性疟原虫对4种常用抗疟药的敏感性  

The Microfluorimetric Assay (MFA) in in vitro Testing the Sensitivity of Plasmodium falciparum to Antimalarial Drugs

作　　者：黄芳[1] 汤林华[1] 王琴美[1] 倪奕昌[1] 

机构地区：[1]中国疾病预防控制中心寄生虫病预防控制所,世界卫生组织疟疾,血吸虫病和丝虫病合作中心,上海200025

出　　处：《中国寄生虫学与寄生虫病杂志》2006年第1期41-44,共4页Chinese Journal of Parasitology and Parasitic Diseases

摘　　要：目的验证微量荧光法(MFA)用于恶性疟原虫体外药物敏感性和抗疟药物筛选的适用性。方法运用MFA测定氯喹、青蒿素、蒿甲醚和咯萘啶等4种常用抗疟药物对体外培养FCC1/HN株恶性疟原虫的敏感性,并对该方法所测得的量效曲线(dose-response curves)及50%有效抑制浓度(IC50)与光学显微镜镜检法所得的结果进行比较。结果MFA所测定的氯喹、青蒿素、蒿甲醚和咯萘啶的IC50分别为18·79、6·32、3·67和2·00nmol/L,光学显微镜镜检法的结果分别为19·65、5·82、4·38和2·83nmol/L,两者差异均无统计学意义(P>0·05)。结论用MFA体外测定恶性疟原虫对抗疟药的敏感性敏感、快速,可用于体外抗疟药物的敏感性测定及抗疟药物筛选。Objective To determine the feasibility of the microfluorimetfic assay （MFA） in screening antimalarial drugs. Methods The dose-response curves and effective concentration that resulted in a 50% inhibitory of parasitemia （IC50） of chloroquine, artemisinin, artemether and pyronaridine phosphate against Plasmodium falciparum strain FCC1/HN cultured in vitro were tested by microfluorimetric assay （MFA） and compared with those determined by microscopy-based assay. Results The IC50 of chloroquine, artemisinin, artemether and pyronaridine phosphate were 18.79, 6. 32, 3.67 and 2.00 nmol/L by MFA and the IC50 of which were 19. 65, 5.82, 4. 38 and 2. 83 nmol/L by microscopy-based technique respectively. The results from both techniques were similar or identical （ P 〉 0. 05）. Conclusion The microfluorimetric assay （MFA） is sensitive, rapid, easily interpreted and would be useful for testing the sensitivity of Plasmodium falciparum to antimalarials and for screening drugs.

关 键 词：抗疟药 恶性疟原虫 药物筛选 微量荧光法 

分 类 号：R382.312[医药卫生—医学寄生虫学]