弓形虫抗原检测方法的研究  

Study on the Methods for Detection of Toxoplasma Antigen

作　　者：李辉[1] 许汴利[1] 邓艳[1] 赵旭东[1] 蔺西萌[1] 闫旭霞[1] 

机构地区：[1]河南省疾病预防控制中心,郑州450003

出　　处：《中国寄生虫学与寄生虫病杂志》2006年第1期67-69,共3页Chinese Journal of Parasitology and Parasitic Diseases

摘　　要：本研究制备了与多种寄生虫抗原无交叉反应的抗弓形虫单抗B6C3和兔抗弓形虫多抗,多抗为捕获抗体,单抗为检测抗体,建立单-多抗夹心ELISA方法。用亲和素连接分别标记生物素的抗体和酶,建立ABC(avidin-biotin-peroxidase complex)-ELISA[D1]方法。同样用亲和素连接标记生物素的抗体和DNA片段,利用PCR反应对DNA片段巨大的扩增能力,提高检测灵敏度,发展单-多抗夹心ELISA为免疫-PCR(I-PCR)检测弓形虫抗原。单-多抗夹心ELISA检测弓形虫抗原最低浓度为0·6μg/ml,ABC-ELISA检测最低浓度为0·075μg/ml。I-PCR检测最低浓度为0·1ng/ml,检测弓形虫抗原灵敏度高于传统ELISA法6000倍,高于ABC-ELISA法750倍。A sandwich ELISA was established with monoclonal antibody as detecting antibody and rabbit antiToxoplasma polyclonal antibody as capturing antibody. ABC （ avidin-hiotin-peroxidase complex）-ELISA and irnmuno-PCR were also used to detect different concentrations of Toxoplasma antigen. The lowest concentration of antigen to be detected by sandwich ELISA, ABC-ELISA and immuno-PCR was 0. 6 μg/ml, 0. 075 μg/ml and 0. 1 ng,/ml respectively. The immuno-PCR shows a much higher sensitivity than the other two methods.

关 键 词：弓形虫 抗原 免疫测定 

分 类 号：R531.8[医药卫生—内科学]