复方虫草制剂延长猪同种异体移植皮存活期的实验研究  

Effects of compound preparation of Cordyceps sinensis and Tripterygium hypoglaucum on survival time of pigskin after allogeneic transplantation

作　　者：程代薇[1] 邹勇[2] 钱宁[3] 王朝亮[2] 田应彪[2] 王达利[2] 赵桂香[1] 高振宇[2] 

机构地区：[1]贵州省人民医院整形烧伤外科,贵州贵阳550002 [2]遵义医学院第一附属医院,贵州遵义563003 [3]贵阳中医学院动物实验中心,贵州贵阳550000

出　　处：《中西医结合学报》2006年第2期185-188,共4页Journal of Chinese Integrative Medicine

基　　金：贵州省科技厅社会攻关项目(No.199971162)

摘　　要：目的:在猪同种异体皮肤移植中,采用由冬虫夏草和昆明山海棠制成的复方虫草制剂(compoundofCordycepssinensisandTripterygiumhypoglaucum,CSTHC)进行移植皮片的孵化和局部外涂,观察CSTHC对移植皮片存活时间的影响,并探讨其作用机制。方法:采用CSTHC水煎剂浸泡孵化供体猪皮片,然后进行皮片移植,局部外涂CSTHC软膏。以环孢素A(cyclosporineA,CsA)和生理盐水作为对照,观察移植皮片的存活情况。移植术前及术后检测外周血白细胞计数、肝肾功能;观察移植皮片及睾丸的组织学表现;采用免疫组织化学法检测各组移植皮片CD4+、CD8+T细胞的表达。结果:CSTHC组移植皮片的存活时间为(28.50±3.26)d,较模型组(10.60±1.52)d明显延长,差异有统计学意义;与CsA组(28.33±3.50)d比较,则无统计学差异。移植术后第7、14天,CSTHC组移植皮片局部CD4+、CD8+T细胞浸润表达低于模型组,差异有统计学意义;与CsA组比较则无统计学差异。移植术后第7天,模型组外周血白细胞计数明显高于CSTHC组及CsA组,差异有统计学意义。结论:局部应用CSTHC可以明显延长移植皮片的存活时间,且无明显的毒副作用。其抑制移植皮片免疫排斥反应的机制可能与减少移植皮片局部炎性细胞尤其是CD4+、CD8+T细胞的浸润有关。Objective： To investigate the effects of compound preparation of Cordyceps sinensis and Tripterygium hypoglaucum （CSTHC） on survival time of grafted pigskin after allogeneic transplantation and its mechanism. Methods： The pigskin was treated with CSTHC solution before allogeneic transplantation, and CSTHC ointment was applied for external use on the grafted pigskin after skin transplantation. Cyclosporine A （CsA） and normal saline were served as control. The survival time, the appearance and the histomorphologi-cal changes of the grafted pigskin were observed. The histomorphological changes of testicles in pigs were also examined. The CD4 and CD8 expressions in the grafted pigskins were measured by immunohistochemical method. The white blood cell count in peripheral blood and the liver and renal functions were also examined. Results： The survival time of the grafted pigskin in the CSTHO-treated group was （28.50 ± 3.26） d, whichwas much longer as compared with （ 10.60 ± 1.52） d in the untreated group （ P〈0.01 ）. The surwvai time of the grafted pigskin in the CsA-treated group was （28.33 ± 3.50） d, and there was no remarkable difference in the survival time of the grafted pigskin between the CsA-treated group and the CSTHO-treated group. The expressions of CD4 and CD8 were lower in the CSTHO-treated group than those in the untreated group on the 7th and 14th day after skin graft （P〈0.05）, while there was no significant difference in the indices between the CSTHO-treated group ancl the CsA-treated group. The WBC count was higher in the untreated group than that in the CSTHO-treated group or CsA-treated group on the 7th day after skin graft （P〈0.05）. Conclusion： OSTHO can prolong the survival time of allogeneic grafted pigskin. Its mechanism of inhibiting the immunolog-ical rejection may relate to-decreasing the expressions of OD4^＋ and OD8^＋ in the grafted pigskin and reducing the local inflammatory reaction.

关 键 词：冬虫夏草 同种异体移植 皮肤移植 组织存活 免疫排斥 

分 类 号：R285.5[医药卫生—中药学]