Influence of Ciglitazone on A549 Cells Growth in vitro and in vivo and Mechanism  

作　　者：张万广 张惠兰 邢丽华 

机构地区：[1]Hepatic Surgery Center,Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China [2]Department of Respiratory Diseases, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China

出　　处：《Journal of Huazhong University of Science and Technology(Medical Sciences)》2006年第1期36-39,共4页华中科技大学学报（医学英德文版）

基　　金：ThisprojectwassupportedbyagrantfromNationalNatu-ralSciencesFoundationofChina(No.30500224).

摘　　要：The effect and mechanism of the ciglitazone on lung cancer cells A549 growth in vitro and in vivo were studied. Various concentrations of ciglitazone were added to the cultured A549 line, and the proliferation and differentiation of A549 cells were examined by MTT and cytometry analysis. A549 cells （1 × 10^6/mouse） were inoculated subcutaneously into 20 nude mice, which were randomly divided into two groups., the control group, the ciglitazone treated group. The weights of subcutaneous tumors were measured, The expression of cyclin D1 and P21 in the lung was detected by immohistochemistry and Western blot respectively. The results showed that the proliferation of A549 was inhibited significantly by ciglitazone in a dose- and time-dependent manner. There were more ceils arrested in G1/G0 phase and the expression of PPARγ was markedly upregulated in ciglitazone-treated group. Direct injection of ciglitazone into A549-induced tumors could suppress tumor growth in nude mice and the growth inhibitory rate was 36 %. The expression of cyclin D1 was decreased and P21 increased significantly in ciglitazone-treated group as compared with control group. It was concluded that ciglitazone could inhibit A549 proliferation dose-dependently and time-dependently and induce differentiation, ,which might be related to the modulation of cell cycle interfered by PPARγ.The effect and mechanism of the ciglitazone on lung cancer cells A549 growth in vitro and in vivo were studied. Various concentrations of ciglitazone were added to the cultured A549 line, and the proliferation and differentiation of A549 cells were examined by MTT and cytometry analysis. A549 cells （1 × 10^6/mouse） were inoculated subcutaneously into 20 nude mice, which were randomly divided into two groups., the control group, the ciglitazone treated group. The weights of subcutaneous tumors were measured, The expression of cyclin D1 and P21 in the lung was detected by immohistochemistry and Western blot respectively. The results showed that the proliferation of A549 was inhibited significantly by ciglitazone in a dose- and time-dependent manner. There were more ceils arrested in G1/G0 phase and the expression of PPARγ was markedly upregulated in ciglitazone-treated group. Direct injection of ciglitazone into A549-induced tumors could suppress tumor growth in nude mice and the growth inhibitory rate was 36 %. The expression of cyclin D1 was decreased and P21 increased significantly in ciglitazone-treated group as compared with control group. It was concluded that ciglitazone could inhibit A549 proliferation dose-dependently and time-dependently and induce differentiation, ,which might be related to the modulation of cell cycle interfered by PPARγ.

关 键 词：peroxisome proliferator activated receptor γ CIGLITAZONE cyclin D1 P21 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]