Effects of 17β-Estradiol on the Expression of IL-6, IL-11 and NF-κB in Human MG-63 Osteoblast-like Cell Line  

作　　者：王运林 廖二元 向光大 戴如春 肖新华 罗湘杭 

机构地区：[1]Department of Gerontism, Tongji Hospital, Tongji Medical College, Huazhong university of Science and Technology, Wuhan 430030, China [2]Institute of Endocrinology and Metabolism, Second Xiangya Hospital, Central South University, Changsha 410011, China [3]Department of Endocrinology, Wuhan General Hospital of PLA, Wuhan 430070, China

出　　处：《Journal of Huazhong University of Science and Technology(Medical Sciences)》2006年第1期53-58,共6页华中科技大学学报（医学英德文版）

基　　金：ThisprojectwassupportedbyagrantfromNationalNatu-ralSciencesFoundationofChina(No.39970347).

摘　　要：In order to characterize the effects of 17β estradiol （17β-E2） on the expression of IL-6, IL-11 and NF-κB in the human MG-63 osteohlast-like cell line, the expression of IL-6 was detected by RT-PCR, Northern blot and Western blot. The expression of IL-11 was determined by RTPCR, and NF-κB by Western blot. The results showed that 17β-E2 down-regulated the expression of IL-6 mRNA and protein, IL-11 mRNA and NF-κB protein in MG-63 cells. It was suggested that the expression of NF-κB, IL-6 and IL-11 in MG-63 cells could be suppressed by 17β-E2, and this might lend support to estrogen replacement therapy in postmenopausal women.In order to characterize the effects of 17β estradiol （17β-E2） on the expression of IL-6, IL-11 and NF-κB in the human MG-63 osteohlast-like cell line, the expression of IL-6 was detected by RT-PCR, Northern blot and Western blot. The expression of IL-11 was determined by RTPCR, and NF-κB by Western blot. The results showed that 17β-E2 down-regulated the expression of IL-6 mRNA and protein, IL-11 mRNA and NF-κB protein in MG-63 cells. It was suggested that the expression of NF-κB, IL-6 and IL-11 in MG-63 cells could be suppressed by 17β-E2, and this might lend support to estrogen replacement therapy in postmenopausal women.

关 键 词：IL-6 IL-11 NF-ΚB MG-63 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]