弥漫性大B细胞淋巴瘤bcl-2/IgH基因重排与GCB分子亚型的相关性  

Correlation between bcl-2/IgH gene rearrangement and GCB molecular subtype in diffuse large B-cell lymphoma

作　　者：蒋会勇[1] 陈愉[1] 张三泉[1] 朱梅刚[1] 赵彤[1] 

机构地区：[1]南方医科大学附属南方医院病理科,广东广州510515

出　　处：《第四军医大学学报》2006年第5期420-423,共4页Journal of the Fourth Military Medical University

基　　金：广东省社会发展攻关项目(B30301);广州市科技计划项目(2002Z34061)

摘　　要：目的:探讨弥漫性大B细胞淋巴瘤(DLBCL)bcl-2/IgH基因重排与分子亚型生发中心样(GCB)的相关性.方法:采用自行设计的半巢式PCR对60例DLBCL的bcl-2/IgH基因重排进行了检测,并对阳性产物进行克隆、测序.同时采用免疫组化SP法在组织微阵列上同步观测CD20,CD10,Bcl-6,黑色素瘤相关抗原(突变体)1(MUM1)的表达,进行GCB和非生发中心样(non-GCB)分子分类.结果:经常规法扩增bcl-2/IgH,有6/60例DLBCLbcl-2/IgH阳性;在6例阳性样本中,采用本组设计的半巢式PCR扩增,经克隆及测序证实5例bcl-2/IgH基因重排阳性,1例为人类第19号染色体BAC331191基因的片段.60例DLBCLCD20表达全部阳性;CD10,Bcl-6,MUM1的阳性表达率分别为43.3%,46.7%,61.7%;GCB32(53.3%)例,non-GCB28(46.7%)例.bcl-2/IgH基因重排阳性的病例均属GCB分子亚型,且与CD10表达有显著性相关(P=0.012),而与Bcl-6及MUM1表达无相关性.结论:使用本组设计的半巢式PCR可提高bcl-2/IgH基因重排检测的准确性.bcl-2/IgH基因重排的检测可协助确定部分GCB分子亚型的DLBCL.AIM： To explore the correlation between bcl-2/IgH gene rearrangement and germinal center B-cell-like （GCB） molecular subtype in diffuse large B-cell lymphoma （DLBCL）. METHODS： Self-designed heminested PCR was used to detect bcl-2/IgH gene rearrangement in 60 cases of DLBCL. Positive PCR products were cloned and sequenced. The tissue microarray of 60 specimens of DLBCL was prepared and the expressions of CD20, CD10, Bcl-6 and MUM1 were investigated by immunohis tochemical SP method. GCB and non-germinal center B-cell-like （non-GCB） were subclassified. RFSULTS： Six of the 60 DLBCL were bcl-2/IgH positive by conventional PCR method. Self designed heminested PCR was used to amplify 6 positive cases again and bcl-2/IgH gene was verified rearrangement in 5 cases by cloning and sequencing and 1 had the gene segment of BAC331191 of human chromosome 19. CD20 was positive in the 60 DLBCL cases. Positive expression rates of CD10, Bcl-6 and MUM1 were 43. 3%, 46. 7% and 61. 7% respectively. 32 （53.3%） were GCB and 28 （46.7%） were non-GCB. Bcl-2/ IgH gene rearrangement-positive cases were all GCB subtypes, which were significantly correlated with the expression of CD10 （P=0.012） and not with Bcl-6 and MUM1. CONCLUSION： Self-designed heminested PCR can improve the detection accuracy of bcl-2/IgH gene rearrangement. Bcl-2/IgH gene rearrangement detection helps the DLBCL determination of GCB subtypes.

关 键 词：弥漫性大B细胞淋巴瘤 BCL-2 基因重排 

分 类 号：R392.3[医药卫生—免疫学]