NGF对哮喘小鼠肺及初级传入神经元SH2-Bβ表达的调节作用  

The Regulatory Effect of NGF on the Expression of SH2-Bβ in the Lung and Primary Afferent Neurons of the Asthmatic Mice

作　　者：齐金萍[1] 王巧玲[1] 刘晓湘[2] 曹德寿[2] 方秀斌[2] 

机构地区：[1]沈阳医学院解剖学教研室 [2]中国医科大学神经生物学教研室,辽宁沈阳110001

出　　处：《解剖科学进展》2006年第1期23-26,i0002,共5页Progress of Anatomical Sciences

基　　金：国家自然科学基金资助(No.30570811)

摘　　要：目的探讨NGF对哮喘小鼠肺及初级传入神经元(C7-T5脊神经节及相应脊髓后角)SH2-Bβ表达的调节作用。方法BALB/c小鼠30只,按随机数字表法分为正常对照组、哮喘组、anti-NGF组,每组10只。利用免疫组织化学方法,免疫印迹法(W estern B lot)测定各组SH2-Bβ的免疫反应变化;M etamoph图象分析系统对结果进行分析。结果免疫组织化学结果显示哮喘小鼠肺、C7-T5节段脊神经节及对应的脊髓后角SH2-Bβ免疫阳性产物平均光密度值(MOD)分别为0.806±0.023、0.766±0.018、0.547±0.014,明显高于对照组(0.243±0.018、0.131±0.011、0.215±0.029)(P<0.01)。而Anti-NGF组小鼠SH2-Bβ免疫阳性产物MOD分别为0.252±0.015、0.158±0.012、0.251±0.024,明显低于哮喘组(P<0.01)。W estern B lot显示哮喘小鼠的C7-T5节段脊髓及肺内SH2-BβMOD与-βactin MOD的比值(0.738±0.021和1.526±0.022)明显高于对照组(0.346±0.017和0.512±0.018,P<0.01);而Anti-NGF组小鼠在肺、C7-T5节段脊髓SH2-Bβ免疫阳性产物MOD与β-actioin MOD的比值(0.436±0.011和0.682±0.015)明显低于哮喘组(P<0.01)。结论肺内、C7-T5脊神经节及对应脊髓后角神经元的SH2-Bβ可能参与NGF介导的哮喘的发病过程。Objective To explore the regulatory effect of NGF on the expression of SHZ-Bβ in the lungs, primary afferent neurons（ C7 -T5 spinal ganglia and the corresponding posterior horn of the spinal cord） of the asthmatic mice. Methods 30 BALB/c mice were randomly divided into normal control group , asthmatic group and anfi-NGF group on average. The alterations of SH2-Bβ immunoreactivity were investigated by immunohistochemi and Western blot methods and analyzed by Metamoph analysis system. Results Immunohistochemistry demonstrated that the mean optic density（MOD） of SH2-B[3 positive substance in the lungs（0. 806 ±0.023）, CT-T5 spinal ganglia（0.766 ± 0.018） and the corresponding posterior horn of the spinal cord （0.547 ± 0.014） of the asthmatic mice was much higher than those （ 0. 243 ± 0. 018,0.131 ±0. 011, 0. 215 ± 0. 029 ） in normal control group respectively （ P 〈 0. 01 ）, but MOD values （0.252 ± 0.015 ,0.158 ± 0.012,0.251 ± 0.024 ） in anti-NGF group are much lower than those in the asthmatic mice（ P 〈 0.01 ）. Western Blot demonstrated that the mean ratio of SH2-Bβ MOD to β-actin MOD in spinal cord and lung was 0. 738 ± 0. 021 and 1. 526 ± 0. 022 in asthmatic group, much higher than 0.346 ±0.017 and 0.512 ± 0.018 in normal control group （ P 〈 0.01 ） ; and those （ 0.436 ± 0.011 and 0. 682 ± 0.015 ） in anti- NGF group were much lower than those of asthmatic mice（ P 〈 0.01 ）. Conclusion The present results indicate that SH2-Bβ in the lungs, primary afferent neurons（ C7-T5 spinal ganglia and the corresponding posterior horn of the spinalcord） might be involved in the pathogenesis of NGF mediated asthma.

关 键 词：SH2-BΒ 神经生长因子 脊髓 脊神经节 哮喘小鼠 

分 类 号：R562.25[医药卫生—呼吸系统] R322.85[医药卫生—内科学]