根癌农杆菌介导的番茄遗传转化  

The Genetic Transformation of Tomato Mediatied by Agrobacterium tumefaciens

作　　者：任永霞[1] 王罡[2] 季静[2] 王萍[3] 

机构地区：[1]河北北方学院牧业工程系,河北张家口075000 [2]天津大学农业与生物工程学院,天津300072 [3]淮海工学院海洋学院,江苏连云港222005

出　　处：《河北北方学院学报（自然科学版）》2005年第5期35-38,共4页Journal of Hebei North University:Natural Science Edition

基　　金："国家植物转基因技术研究开发与中试基地建设"专项课题(J99-B-001)

摘　　要：利用根癌农杆菌(Agrobacterium tumefaciens)EHA101携带LycB基因(番茄红素β-环化酶)和潮霉素抗性基因,以东农704、708、709三个番茄品种的子叶、茎段为转化受体对番茄进行遗传转化,经PCR分子检测初步证明,目的基因LycB已整合进番茄基因组中。Mediated by Agrobacterium turnefaciens EHA101 with Target LycB gene and resistant gene to Hygromycin, tomato species including Dongnong 704, 708, 709 being used as material and their cotyledons, petiol segments as acceptor, the genetic transformation of tomato was studied. It was prilminarily proved by the PCR examination that target LycB gene was integrated into the genome of tomato.

关 键 词：根癌农杆菌 番茄 LycB基因 遗传转化 

分 类 号：S641.2[农业科学—蔬菜学] S188[农业科学—园艺学]