转基因与常规杂交相结合改良水稻耐盐性  

Improvement of Rice Salt-Tolerance by Using an Integrated Method of Gene Transformation and Traditional Breeding

作　　者：郭龙彪[1] 薛大伟[1] 王慧中[1] 陈受宜[2] 卢德赵[1] 曾大力[1] 高振宇[1] 颜美仙[1] 黄大年[1] 钱前[1] 

机构地区：[1]中国水稻研究所水稻生物学国家重点实验室 [2]中国科学院遗传与发育生物学研究所,北京100101

出　　处：《中国水稻科学》2006年第2期141-146,共6页Chinese Journal of Rice Science

基　　金：国家863计划资助项目(JY03-B-8);国家自然科学基金资助项目(302376)。

摘　　要：通过农杆菌介导法和基因枪法将CMO、BADH、muD、gutD和SAMDC基因以单价或双价的形式导入水稻常规品种中，再结合常规杂交育种，选育5价强耐盐性的转基因水稻植株。1～5价9类组合的水稻品系，经PCR分子检测．在转基因后代中多价目的基因聚合，遗传稳定，且分子检测与田间耐盐性的表现一致。转基因植株在盐碱地中能正常生长，拓展了水稻常规品种耐盐性。并已获得耐0．5％～1．0％NaCI的T秀水11——品3、品6和品7等9份优良株系或中间材料。The genes encoding enzymes responsible for synthesizing some low-molecular weight metabolites, such as betaine, polyamines, and mannitol,were transformed into rice plants to enhance the salt-tolerance. The improvement of rice salt tolerance with five target genes （BADH, CMO, mtlD, gutD and SAMDC） was conducted through gene transformation and traditional breeding approaches. Results indicated that the genes were successfully transformed into rice by particle bombardment or mediated by Agrobacteriurn turnefaciens and integrated into the genome of the transgenic plants by using PCR detections. The salt tolerance of the transgenic plants and their progenies with 1 5 genes was significantly enhanced and stably maintained in the salt-stress fields. Nine elite rice transgenic lines such as Pin 3, Pin 6 and Pin 7 from Xiushui 11 were obtained, which grew normally in the salt pool containing 0.5%- 1.0% NaCl.

关 键 词：耐盐相关基因 遗传转化 常规杂交 新种质 水稻 

分 类 号：S511.22[农业科学—作物学]