转溶菌酶基因水稻回交转育籼型杂交稻亲本  

Transfer of Lysozyme Gene into indica Parents of Hybrid Rice by Backcrossing

作　　者：易自力[1] 王紫萱[1] 覃静萍[1] 蒋建雄[1] 谭炎宁[1] 周清明[2] 

机构地区：[1]湖南农业大学细胞工程重点实验室,湖南长沙410128 [2]湖南农业大学农学院,湖南长沙410128

出　　处：《中国水稻科学》2006年第2期147-152,共6页Chinese Journal of Rice Science

基　　金：湖南省教育厅科学研究重点资助项目(03A016);教育部科学研究重点资助项目(204016);湖南省农大科技专项子项目(04NK1002)。

摘　　要：以抗稻瘟病的粳稻中花9号转溶菌酶基因材料D2-1-2与籼型杂交稻两优培九的恢复系9311及不育系培矮64S、汕优63的恢复系明恢63分别杂交和多代回交,进行外源溶菌酶基因的转育。对获得的转育回交后代B39311、B3MH63、B2PA64S和回交自交后代B2F29311、B2F2MH63、B1F2PA64S进行了PCR分析,表明外源基因在回交后代中呈1∶1分离,而在回交的自交后代中呈3∶1分离,证明外源溶菌酶基因是以单拷贝稳定传递给后代的。2003年和2004年对转育后代和测交组合进行了稻瘟病抗性调查,结果表明,转育后代抗性与轮回亲本相比、测交组合抗性与对应杂交稻组合相比都有了较大提高。随着转育回交世代的增加,抗性增强得越明显。研究表明通过回交转育方法将外源溶菌酶基因导入杂交稻亲本是选育抗稻瘟病杂交稻新组合的一条简便有效的途径。A lysozyme gene resistant to rice blast from the donor D2-1-2, a transgenic japonica rice line of Zhonghua 9, was transferred into the sterile line Pei＇ai 64S（PA64S） and restorer line 9311 of the hybrid Liangyoupeijiu, and the restorer line Minghui 63 （MH63） of Shanyou 63 by successive backcrosslng. PCR analysis confirmed that foreign lysozyme gene was segregated with the ratio of 1 ; 1 in backcross generations of Ba 9311, B3 MH63 and Be PA64S, and with the ratio of 3 ： 1 in inbred generations of B2 F2 9311, B2 F2 MH63 and B1 F2 PA64S, indicating the foreign gene was stably inherited over successive generations as a dominant single copy gene. The resistance to rice blast of the backcross or inbred generations and corresponding testcross combinations were investigated in the years of 2003 and 2004, and the results showed that their resistance to rice blast had a greater improvement than that of the corresponding recurrent parents or the corresponding hybrid combinations, respectively. The resistance to rice blast became stronger with the generations of the transferred rice progressed. This con firmed that transferring the lysozyme gene into hybrid parents by backcrossing was a simple and effective approach to develope new hybrid combinations resistant to rice blast.

关 键 词：杂交稻 溶菌酶基因 稻瘟病 抗性 回交 育种 

分 类 号：S511.21[农业科学—作物学]