水稻红莲型细胞质雄性不育幼穗发育过程中组织型转谷氨酰胺酶活性比较  

Comparison of Tissue Transglutaminase Activity During Young Panicle Development in Honglian-Type Cytoplasmic Male Sterile Rice (Oryza sativa)

作　　者：常逊[1] 张再君[1] 李阳生[1] 朱英国[1] 

机构地区：[1]武汉大学生命科学学院植物发育生物学教育部重点实验室,湖北武汉430072

出　　处：《中国水稻科学》2006年第2期183-188,共6页Chinese Journal of Rice Science

基　　金：国家973计划资助项目(2001CB108806);创新研究群体科学基金资助项目(C01020305);长江学者和创新团队发展计划资助项目。

摘　　要：以水稻红莲型细胞质雄性不育系粤泰A和保持系粤泰B的叶片和不同发育时期幼穗为材料,比较分析了水稻红莲型细胞质雄性不育幼穗发育过程中组织型转谷氨酰胺(tTG)酶活性变化,建立了适合水稻tTG酶活性分析的酶联免疫法(ELISA)测定反应体系。研究发现tTG酶活性受钙离子正调控,并且无论是粤泰A还是粤泰B,衰老叶片中tTG酶活性都高于新叶片,但粤泰A与粤泰B之间的差异不明显;不育系粤泰A自四分体到二核期的幼穗不同发育阶段,tTG酶活性随着花粉发育而增强,在二核期达到最高,而在保持系粤泰B中,tTG酶活性没有随发育进程发生显著变化。推断tTG酶与花粉败育过程中的细胞程序性死亡有关。The leaves and young panicles at different developmental stages, being excised from the Honglian-type cytoplasmic male sterile line, Yuetai A and its maintainer line, Yuetai B, were used to investigate the changes of tissue transglutaminase activity. An EL1SA （enzyme linked immunosorbnent assay） measurement protocol for tissue transglutaminase activity detection in rice was well established. It was found that tissue transglutaminase activity was regulated positively by calcium cation, and in Yuetai A and Yuetai B the tissue transglutaminase activity in the senescent leaves was remarkably higher than that in the young leaves, but there was no distinct difference between Yuetai A and Yuetai B. Interestingly, from the tetrad to binucleate stages the tissue transglutaminase activity increased gradually with the development of the young panicles and up to maximum at binucleate stage in Yuetai A. However, no similar changes were observed in Yuetai B. It was sugges ted that tissue transglutaminase was involved in programmed cell death in abortive pollen.

关 键 词：水稻 细胞质雄性不育 幼穗发育 组织型转谷氨酰胺酶 细胞程序性死亡 

分 类 号：S511[农业科学—作物学]