长春新碱载体红细胞天然免疫活性及其分子变化研究  

Innate immune adherence activity to tumor cells and phenotype properties of Vincristine-loaded intact human erythrocytes

作　　者：钱宝华[1] 吴江[1] 杨林军[2] 郭峰[1] 牛芳[1] 罗庆峰[1] 

机构地区：[1]第二军医大学长海医院输血科,上海200433 [2]第二军医大学长海医院肿瘤科

出　　处：《临床血液学杂志》2006年第2期79-81,共3页Journal of Clinical Hematology

摘　　要：目的:研究长春新碱(VCR)载体红细胞的天然免疫黏附活性变化,并从分子水平进行深入探讨。方法:改良低渗预膨胀法制备VCR载体红细胞。采用新鲜血天然免疫功能快速测定法和荧光标记流式细胞分析的方法,检测载体制备过程中洗涤、预膨胀和载药各阶段红细胞的天然免疫黏附活性及相关表面分子的变化情况。结果:低渗预膨胀处理的肿瘤患者红细胞,天然免疫黏附能力[黏附率(39.20±18.14)%]明显高于其他对照组(P<0.05)。健康人红细胞载药前后天然免疫黏附能力[黏附率(48.30±13.52)%和(51.20±11.24)%],均明显高于肿瘤患者(P<0.05),但VCR载药对红细胞的天然免疫黏附活性影响不明显。洗涤、低渗预膨胀和载药处理后,红细胞表面CD35、CD44、CD55、CD59等分子有不同程度的下降,CCR4分子呈上升趋势。结论:载药处理对红细胞天然免疫黏附活性无明显影响。Objective：To study the effect of VCR loaded process on innate immune adherence activity to tumor cells of erythrocytes and explore its molecule mechanism preliminary, Method：VCR was loaded in human erythrocytes using the modified hypotonic preswelling technique. We used the erythrocytes after undergoing washing, swelling and drug-loading treatment from healthy donors and tumor patients, and compared their innate immune adherence activity to tumor cells through measuring the rate of rosette formation and expression of CD35, CD44, CD55, CD59, and CCR4 by flow cytometry. Result： We observed that the innate immune adherence activity of e rythrocytes from tumor patients after hypotonic swelling treatment significantly increased as compared with other groups （ P〈0.05） with. the rate of rosette formation up to （39.20±18.14）%. The innate immune adherence activity of erythrocytes from healthy donors slightly increased after drug-loading. The rates of rosette formation before and after drug-loading were （48.30±13.52）X and （51. 20±11.24）% respectively, both are significant high er than those from tumor patients （ P 〈0.05）. FACS analysis demonstrated that expression of CD35, CD44, CD55, and CD59 on erythrocytes was down regulated after washing, swelling or drug loading treatment, but CCR4 was up-regulated. Conclusion：VCR loaded treatment did not affect the innate immune adherence activity of erythrocytes to tumor cells significantly.

关 键 词：红细胞 药物载体 长春新碱 天然免疫 膜分子 

分 类 号：R392.12[医药卫生—免疫学]