多种细胞因子与急性移植物抗宿主病发病关系的研究  

Study of the relationship between cytokines and aGVHD after allogeneic hematopoietic stem cell transplantation

作　　者：夏凌辉[1] 江汇娟[1] 方峻[1] 刘芳[1] 刘仲萍[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院血液病研究所,武汉430022

出　　处：《临床血液学杂志》2006年第2期90-91,94,共3页Journal of Clinical Hematology

摘　　要：目的：探讨异基因造血干细胞移植（allo-HSCT）患者不同时期血清细胞因子（CK）水平与急性移植物抗宿主病（aGVHD）严重程度的关系。方法：于移植前、移植后2、4、8、12周用流式微球技术监测17例allo—HSCT患者血清细胞因子（TNF-α、IFN-γ、IL-2、IL-4、IL-6及IL-10）水平的动态变化。结果：Ⅱ～Ⅳ度aGVHD患者组（5／17）与0～Ⅰ度aGVHD患者组（12／17）相比：移植后8、12周血清IFN-γ浓度（ng／L）前者较后者显著增高（13．66±1．16，14．10±0．42对比10．00±0．28，11．14±1．13）（P〈0．05）；移植后12周血清IL-6浓度（ng／L）前者较后者显著增高（10．17±1．07对比6．97±1.18，P〈0．05）；其余各时期细胞因子浓度对比无明显差异。结论：异基因造血干细胞移植后，发生Ⅱ～Ⅳ度aGVHD患者较0～1度aGVIID患者血清IFN-γ与IL-6浓度较高，为炎性免疫反应加重表现；TNF-α、IL-2、IL-4及IL-10水平两组间无品著差异，很可能受aGVHD严重程度、免疫抑制剂应用、免疫细胞功能恢复延迟等多因素影响。Objective：To study the serum levels of cytokines in patients after allogeneie hematopoietic stem cell transplantation （allo HSCT） during different periods associated with the grade of acute graft-versus-host disease （aGVHD）. Method：The serum levels of cytokines （TNF-α, IFN-γ, IL-2, IL-4, IL-6, IL-10） were detected in 17 patients by flow cytometric beads assay before and after allo-HSCT on week 2, 4, 8, 12, respectively. Result： Following allo-HSCT 8, 12 weeks later, the serum IFN-γ levels （ng/L） in patients with grade 2-4 aGVHD （13.66±1.16, 14.10±0.42, respectively） were higher than those in patients with grade 0-1 aGVHD （10. 00±0.28, 11.14± 1.13, respectively） （ P 〈0.05）. And following allo-HSCT 12 weeks later, the serum IL-6 levels in patients with grade 2-4 aGVHD were higher than those in patients with grade 0- 1 aGVHD （10.17±1.07 vs 6.97 ±1.18） （ P 〈0.05）. But the levels of TNF-α, IL-2, IL-4 and IL-10 had no differences （ P〈0.05）. Conclusion： After allo-HSCT, the serum levels IFN-γ and IFN-6 in patients with grade 2-4 aGVHD were higher than those in patients with grade 0-1 aGVHD. Levels of TNF-α, IL-2, IL-4 and IL-10 between the two groups had no statistical differences, which may be influenced by grade of aGVHD, administration of immunosuppressive agents and delay of immune function recovery.

关 键 词：细胞因子 干细胞移植 异基因 急性移植物抗宿主病 

分 类 号：R617[医药卫生—外科学]