改良间接MAIPA法对免疫性与非免疫性血小板减少的鉴别诊断价值  

The diagnostic value of platelet glycoprotein-specific autoantibody detection in idiopathic thrombocytopenic purpura

作　　者：肖太武[1] 侯明[2] 李光耀[1] 葛芙蓉[1] 杨锡明[1] 王景霞[1] 栾静[1] 赵歧刚[1] 

机构地区：[1]聊城市人民医院血液科,山东聊城252000 [2]山东大学齐鲁医院血液科

出　　处：《临床血液学杂志》2006年第2期101-102,106,共3页Journal of Clinical Hematology

摘　　要：目的:检测慢性特发性血小板减少性紫癜(CITP)患者及非免疫性血小板减少症患者的抗血小板特异性抗体,评价其诊断价值。方法:建立单克隆抗体特异性俘获血小板抗原技术并加以改进(改良MAIPA法)借以检测CITP患者、慢性再生障碍性贫血(CAA)患者、肿瘤化疗后血小板减少患者抗血小板GPⅡb/Ⅲa、GPⅠb/Ⅸ的特异性抗体。结果:CITP组血小板糖GPⅡb/Ⅲa、GPⅠb/Ⅸ抗体的总阳性率高于CAA组和肿瘤化疗组,且差异有统计学意义(χ2=15.56,P<0.01和χ2=13.02,P<0.01)。结论:在CITP患者中血小板特异性抗体有较高的特异性,具有较高诊断价值,且2种抗体的功效相同。Objective：To evaluate the diagnostic value of platelet glycoprotein specific autoantibody detection between chronic idiopathic thrombocytopenic purpura and other nonimmune thrombocytopenias. Method： Anti-GP Ⅱb/Ⅲa and anti-GPⅠb/Ⅸ autoantibodies were detected by a modified monoclonal antibody immobilization of platelet antigens assay（modified MAIPA）. Result： The total positive rate of antibodies against platelet GP Ⅱb/Ⅲa、GP Ⅰb/Ⅸ in ITP was significantly higher than that in CAA and chemotherapy-induced thrombocytopenias （X^2=15.56, P 〈0.01 X^2= 13.02, P 〈0.01, respectively）. Conclusion：Platelet glycoprotein-speeifie autoantibody had high specificity for the chronic idiopathic thrombocytopenic purpura, and had great value in the diagnosis for immune thrombocytopenias. Anti-GP Ⅱ b/Ⅲ a antibodies had the same value as anti-GP Ⅰ b/IX.

关 键 词：紫癜 血小板减少性 特发性 改良单克隆抗体特异性俘获血小板抗原技术 自身抗体 

分 类 号：R554.6[医药卫生—血液循环系统疾病]