清热解毒和补益中药对小鼠腹水肝癌H_(22)细胞的作用及免疫学机制比较  

Effect of Heat-clearing and Detoxifying Herbs and Tonifying Herbs on Mice Hepatoma Cells Line H_(22) and Their Immunologic Mechanism

作　　者：王洪琦[1] 崔娜娟[1] 胡玲[1] 李建国[1] 胡晨霞[1] 成晓燕[2] 

机构地区：[1]广州中医药大学,广州510405 [2]广东医学院,湛江524023

出　　处：《广州中医药大学学报》2006年第2期156-159,共4页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：广东省自然科学基金项目(编号:020779)

摘　　要：【目的】比较清热解毒中药和补益类中药对小鼠腹水肝癌H22细胞的作用及免疫学机制。【方法】选择冬凌草、半枝莲为清热解毒中药代表药,并与补益代表药黄芪进行比较;将50只KM小鼠随机分为模型组、冬凌草组、半枝莲组、黄芪组和热休克处理对照组(热休克组)。在活体肝癌H22腹水细胞腹腔接种后当天,冬凌草组、半枝莲组和黄芪组分别以30、 15、15 g·kg-1·d-1的剂量灌胃给药,连续12 d;热休克组小鼠以43℃热处理20 min,每4天1次,共3次;模型组不予任何处理。12 d后处死小鼠,分别抽取腹水制片,光镜下观察细胞形态,并采用免疫组化法检测腹水CD4+、CD8+T细胞表达。【结果】冬凌草组、半枝莲组和热休克组见腹水肝癌H22细胞大量坏死和凋亡,黄芪组对肿瘤细胞形态无明显影响;冬凌草组和半枝莲组腹水中CD4+、CD8+表达均升高,而热休克组则主要是CD8+表达升高,黄芪组以CD4+表达升高为主。【结论】清热解毒中药可致小鼠腹水肝癌H22细胞坏死和凋亡,机制可能与其提高细胞免疫功能,从而增强机体对肿瘤细胞的免疫杀伤能力有关。黄芪未见有直接杀伤肿瘤细胞的作用,其抗肿瘤作用可能主要是通过提高CD4+的表达而实现的。[ Objective] To compare the effects of heat-clearing and detoxifying herbs with that of tonifying herbs on mice hepatoma cells line H22, and to explore their immunologic mechanisms. [ Methods ] Herba Rabdosiae Rubescentis （HRR） and Herba Scutellariae Barbatae （HSB） were selected as the heat-clearing and detoxifying herbs, and Radix Astragali （RA） as the representative of tonifying herbs. Fifty KM mice were inoculated with hepatoma cells line H22 and then were randomized into five groups： HRR group （30 g· kg^-1·d^-1 by garage for 12 days）, HSB group （15 g· kg^-1· d^-1 by garage for 12 days）, RA group （15 g· kg^-1· d^-1 by garage for 12 days）, heat-shock group （treated 20 rain at 43℃, once every 4 days and three times in all） and model group. Twelve days later, the mice were executed and the ascites smears were prepared. The cell feature in ascites was observed under light microscope and the expression of CD4 ^＋ and CD8^ ＋ on the cellular membrane of the T lymphocyte in ascites was detected with immunohistochemical method. [Results] A large amount of damaged and apoptotic H22 cells were found in HRR, HSB and heat-shock groups, and the changes of cell feature in RA group were not obvious. The expressions of CD4^＋ and CD8^ ＋ on the membrane of T cells in ascites was increased in HRR and HRB groups, but the expression of CD4^ ＋ was increased in RA group, and CD8^ ＋ expression was increased in heat-shock group. [ Conclusion] Heat-clearing and detoxifying herbs can induce the necrosis and apoptosis of mice hepatoma cells line H22 and the mechanism may be related to the increase of cellular immunity, thus enhancing the body immunity to kill the tumor cells. RA can not kill the tumor cells directly and its antitumor action may be related to the increase of CD4^＋ expression.

关 键 词：冬凌草/药理学 半枝莲/药理学 黄芪/药理学 肿瘤/中药疗法 肿瘤/免疫学 疾病模型 动物 小鼠 

分 类 号：R285.5[医药卫生—中药学]