大黄对大鼠肝再生过程中信号调节蛋白α1的影响  

Effect of Rhubarb on expression of signal regulatory protein α1 in hepatic regenerating rat

作　　者：朱清静[1] 陈锋[2] 盛国光[1] 

机构地区：[1]湖北省中医院肝病诊疗中心,湖北武汉430061 [2]湖北中医学院

出　　处：《中西医结合肝病杂志》2006年第1期21-22,共2页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

基　　金：湖北省自然科学基金课题资助(No.2004ABA236)

摘　　要：目的：观察大黄对大鼠肝再生过程中信号调节蛋白α1（SIRPα1）的表达变化。方法：雄性wistar大鼠24只，体质量230～250g，随机均分为正常组、模型组、犬黄组和PHGF组，每组6只，大黄组和PHGF组动物于实验前3天至实验结束时分别于皮下注射大黄注射液1ml／100g和促肝细胞生长素（PHGF）lml／100g，对照组和模型组大鼠同时皮下注射0．85％氯化钠液1ml／100g。除正常组大鼠仅进行肝叶的牵拉，其余大鼠均进行70％肝叶切除复制模型。48小时后，杀死动物，迅速取肝组织，用10％甲醛液固定，石蜡切片，检测SIRPα1的表达。结果：大黄可增强大鼠肝再生过程中SIRPα1的表达（P〈0．01）。结论：大黄通过影响SIRPα1而参与肝再生的过程。Objective： To observe the effect of Rhubarb on expression of signal regulatory protein αl in hepatic regenerating rats.Methods： Twenty four rats were randomly divided into normal group, model group, Rhubarb group and PHCF （lyophilized bovine transfer factor） control group. Medication rats were given 1 ml/100g Rhubarb or PHGF injection respectively 3 days before hepatectomy once a day. At the same time, the rats of normal group and model group were hypodermically in jected with the 0.85% salt solution. Except the normal group, 70% of liver including medial and left lobes were ,sectioned. Forty eight hours later, all animals were sacrificed, hepatic tissues were fixed and embedded into paraffin and sectioned. The expression of signal regulatory protein α1 in hepatic tissue was determined by immunohistochemistry. Results： Rhubarb can obviously proliferation signal regulatory protein α1 in hepatic regenerating rats comparing with model group and control group, there were significant difference （P 〈 0.01 ） . Conclusion： Rhubarb has the function of regulation the expression of signal regulatory protein α1 in hepatic regenerating rats, improve liver regeneration.

关 键 词：肝再生 信号调节蛋白α1 大黄/药效学 大鼠 

分 类 号：R285.5[医药卫生—中药学]