酶法拆分DL-茶氨酸及其分离纯化  

Resolution of DL-theanine by Aminoacylase and the Separation of Productions

作　　者：郭丽芸[1] 刘毅[1] 贾晓娟[1] 李兆兰[1] 焦庆才[1] 

机构地区：[1]南京大学医药生物技术国家重点实验室,江苏南京210093

出　　处：《茶叶科学》2006年第1期31-36,共6页Journal of Tea Science

基　　金：国家技术创新基金(02CJ-13-01-16)

摘　　要：将DL-茶氨酸乙酰化为N-乙酰-DL-茶氨酸,利用本实验室筛选的有较高氨基酰化酶活性的真菌刺孢小克银汉霉9980(Cunnighamella echinulata 9980)对DL-茶氨酸进行拆分并对拆分条件进行摸索。结果表明:反应最适温度50℃,最适pH7.0,底物浓度0.5mol/L,湿菌体量4g/100ml,拆分时间30h,拆分率可达92%。产物经JK008阳离子交换树脂氨性柱分离,L-茶氨酸收率84.3%,[α]2D5=8.1(c=2,H2O),符合JP2000药典。An enzymatic method for the optical resolution of DL-theanine by aminoacylases from Cunnighamella echinulata 9980 was developed. The optimum temperature, pH and substrate concentration were： 50%, pH7.0, and 0.Smol/L N-Ac-DL-theanine. The conversion rate against N-Ac-L-theanine was 92%. Productions were purified on JK008 ion exchange resin. The yield of L-theanine was 84.3%, [α]^25D=8.1 （c = 2, H2O）, complied with JP2000.

关 键 词：L-茶氨酸 N-乙酰-DL-茶氨酸 刺孢小克银汉霉9980 氨基酰化酶 光学拆分 

分 类 号：TQ914.1[化学工程]