RNAi技术在凋亡研究中的应用  

The Application of the RNA Interference in Apoptosis

作　　者：黄璐[1] 李新平[1] 霍黉琦[1] 

机构地区：[1]苏州大学生命科学学院,江苏苏州215006

出　　处：《医学综述》2006年第6期321-323,共3页Medical Recapitulate

基　　金：国家自然科学基金资助项目(30471250)

摘　　要：RNA干扰(RNAi)是由双链RNA(dsRNA)引发的转录后基因沉默(PTGS)。dsRNA经Dicer酶降解成21-23nt的siRNA,并以其为模板,特定位点、特定间隔地降解与之序列相应的mRNA。随着RNAi机制的深入研究与广泛应用,目前该技术已经普遍应用于凋亡研究中,在阐明启动与调控凋亡机制的同时也为基因治疗提供了新靶点。RNA interference is a mechanism of post-transcriptional gene silencing （PTGS） induced by doublestranded RNA. dsRNA is firstly degradated into 21-23nt siRNA by Dicer RNase, and then makes itself as a template,trlggers the mRNA that specifical to the model sequence-to degradate at the specific site and the specific spacing. Along with the deep study and the wide application of RNAi ,now this strategy has been applied in apoptot- ie research, it can not only elucidate various mechanisms about priming and regulation of apoptosis, but also supply several new targets for gene therapy.

关 键 词：RNA干扰 凋亡 基因沉默 

分 类 号：Q78[生物学—分子生物学] Q291