脑源性神经营养因子和神经生长因子对顺铂诱导的内耳毒性的保护作用  

Protective Role of BDNF and NGF Against Cisplatin-Induced Ototoxicity

作　　者：张青松[1] 葛圣雷[1] 谢鼎华[1] 朱纲华[1] 

机构地区：[1]中南大学湘雅二医院耳鼻咽喉-头颈外科,中南大学耳科研究所

出　　处：《听力学及言语疾病杂志》2006年第2期130-131,T0002,共3页Journal of Audiology and Speech Pathology

摘　　要：目的研究豚鼠耳蜗内脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)和神经生长因子(nerve growthfactor,NGF)在顺铂致耳中毒后的表达,并探讨其对内耳的保护作用。方法将豚鼠分成对照组、顺铂组,分别给生理盐水、顺铂腹腔注射,于注射后3、5、7天取豚鼠耳蜗行石蜡切片,进行BDNF和NGF免疫组织化学染色,观察螺旋神经节BDNF和NGF蛋白表达。结果对照组耳蜗螺旋神经节细胞中BDNF几乎不表达,NGF呈中度阳性表达。顺铂组BDNF在3天时达到高峰,以后减弱;NGF在5天时达到高峰,以后减弱。结论正常豚鼠耳蜗有中度NGF的表达,表明NGF可能对内耳的正常生理功能起重要作用。顺铂干预后螺旋神经节BDNF和NGF出现高表达,提示BDNF和NGF对顺铂诱导的螺旋神经元损伤有保护作用。Objective To study the expression of brain - derived neurotrophic factor（BDNF） and nerve growth factor （NGF） in the guinea pig cochlea of eisplatin - induced ototoxicity, and to understand its protective role for the inner ear. Mothods The animals were divided into normal group and experimental group with cisplatin. Sodium chloride was used for the control. All animals were sacrificed on the day 3, 5 and 7, and the cochlea were used for BDNF and NGF staining. Results The weak expression of BDNF and the moderate expression of NGF were observed in the cochlea in the normal group. The strongest expression of BDNF and NGF was observed in the animals with cisplatin. Conclusion The existence of NGF in the normal cochlea indicates that NGF plays an important role to maintain auditory physiology. BDNF and NGF may be involved in self- protection mechanism for the cochlea and auditory nerves from cisplatin- induced ototoxicity.

关 键 词：顺铂 脑源性神经营养因子 神经生长因子 

分 类 号：R764.5[医药卫生—耳鼻咽喉科]