丙酮酸乙酯对烫伤延迟复苏大鼠多器官功能及死亡率的影响  

Effect of treatment with ethyl pyruvate on multiple organ dysfunction and mortality following delayed resuscitation after burn injury in rat

作　　者：王文江[1] 姚咏明[1] 咸力明[1] 董宁[1] 于燕[1] 

机构地区：[1]解放军总医院第一附属医院(原解放军第三○四医院)全军烧伤研究所基础部

出　　处：《中国危重病急救医学》2006年第3期132-135,共4页Chinese Critical Care Medicine

基　　金：国家重点基础研究发展规划项目(2005CB522602);国家杰出青年科学基金资助项目(30125020);首都医学发展科研基金重点项目(20032023)

摘　　要：目的观察丙酮酸乙酯(EP)对烫伤延迟复苏动物多器官功能及死亡率的影响,探讨其保护作用的机制。方法采用雄性W istar大鼠30%总体表面积Ⅲ度烫伤模型。实验分为两部分进行:①死亡率观察:130只大鼠按照随机数字表法分为假烫伤组(n=10)、烫伤组(n=60,伤后6 h腹腔注射生理盐水40 m l/kg进行复苏,然后按照不同时间点腹腔注射等量生理盐水)和EP组(n=60,伤后6 h腹腔注射生理盐水40 m l/kg进行复苏,按不同时间点腹腔注射EP液40 m g/kg,每日2次,间隔12 h,给药3 d)。除假烫伤组外,各组又分为伤前2 h(n=20)、伤后2 h(n=20)和伤后12 h(n=20)给药3个亚组。观察不同时间点各组动物7 d死亡率。②器官功能观察:70只大鼠随机分为假烫伤组(n=10)、烫伤组(n=30)和EP组(n=30,伤后2 h给药),并分别于伤后12、24和72 h活杀,检测器官功能指标改变。结果与烫伤组相比,伤后12 h EP组动物死亡率显著降低(35.0%比75.0%,P<0.05)。伤后2 h EP组血清丙氨酸转氨酶、天冬氨酸转氨酶、尿素氮、肌酐、肌酸激酶水平及肺组织髓过氧化物酶活性均明显下降(P<0.05或P<0.01)。结论EP能明显改善严重烫伤延迟复苏大鼠的预后,并对重要器官功能具有显著保护作用。Objective To observe the effects of ethyl pyruvate （EP） treatment on multiple organ dysfunction and mortality following delayed resuscitation after burn injury in rat, and investigate the mechanisms of its protective effect. Methods Male Wistar rats were subjected to 30% full -thickness scald injury followed with delayed resuscitation （6 hours postburn）.①One hundred and thirty male Wistar rats were randomly divided into sham scald group （n-10）, scald group （n=60） and EP- treatment group （n=60）. In the scald group, 40 ml/kg normal saline was injected intraperitoneally 6 hours after scald injury for resuscitation, and it was repeated periodically. Following delayed resuscitation after burn injury, EP was injected at a dose of 40 mg/kg every 12 hours in EP - treatment group for 3 days. According to the interventional time points, rats of scald and EP - treatment groups were respectively divided in three subgroups： 2 hours prior to scald （n=20）, 2 hours after scald （n=20）, and 12 hours after scald （n=20）. The mortality of animals was observed with 7 days as the cut point.②Seventy male rats were randomly divided into sham scald group （n = 10）, scald group （n = 30）, and EP - treatment group （n= 30）. In EP-treatment group, 40 mg/kg EP was injected intraperitoneally 2 hours after scald. Animals were sacrificed at 12, 24, and 72 hours postburn, and serum samples were collected to determine the organ functional parameters, and lung tissue was obtained for measurement of myeloperoxidase （MPO） activity. Results The 7 -day mortality in scald and EP -treatment groups （EP given 12 hours postburn） was 75.0% and 35.0% respectively （P〈0.05）. Compared with scald group, serum alanine aminotransferase （ALT）, aspartate aminotransferase （AST）, blood urea nitrogen （BUN）, creatinine （Cr） and creatine kinase （CK） levels were markedly decreased in EP -treatment group at 12 to 24 hours postburn （P〈0.05 or P〈0.01 ）, and pulmonary MPO activities were

关 键 词：丙酮酸乙酯 烧伤 延迟复苏 多器官功能障碍综合征 死亡率 

分 类 号：R644[医药卫生—外科学]