Galectin-3在糖尿病大鼠肾脏中的表达  

Expression of galectin-3 in diabetic rat kidney

作　　者：楚玉峰[1] 蒋玲[1] 侯志强[1] 张丽萍[1] 张晓黎[1] 

机构地区：[1]山东大学齐鲁医院内分泌科,山东济南250012

出　　处：《山东大学学报（医学版）》2006年第1期60-63,共4页Journal of Shandong University:Health Sciences

摘　　要：目的:探讨不同时期正常和糖尿病大鼠肾组织中galectin-3表达水平的变化及与糖尿病肾病发生发展的关系。方法:2月龄雄性Wistar大鼠60只,随机选取30只,单次腹腔注射链脲佐菌素(STZ,55 mg/kg)诱发糖尿病模型,余30只为正常对照,免疫组化法分别检测4、8、12周末galectin-3抗原在大鼠肾脏中的表达。结果:糖尿病组肾小球8周末开始检测到galectin-3蛋白的产生,且随病程进展有增高趋势。正常组肾小球在4、8、12周末均未见到表达。两组在肾小管组织中,4、8、12周末均可检测到galectin-3的表达,但糖尿病组较同期正常对照组表达显著增高。相关分析表明,糖尿病组肾小球galectin-3阳性细胞平均光密度值与糖化血红蛋白、尿素氮及24 h尿蛋白呈正相关,与肌酐清除率呈负相关。结论:高血糖环境中,galectin-3高表达可能与DN的进展密切相关。Objective： To explore the relationship between the expression of galectin-3 and diabetic nephropathy. Methods： The diabetic model was established in 30 of 2-month male Wistar rats by a single injection of streptozotocin （STZ） at a dose of 55 mg/kg body wt in citrate buffer （pH4.2） and compared with the results from 30 control rats. Semi-quantitative immunohistochemistry was used to observe the expression of galectin-3 4, 8, 12 weeks after diabete induction respectively. Results： Galectin-3 protein had not been detected in glomeruli from nondiabetic rats till the end of study. Excluding at 8 weeks of disease duration, galectin-3 expression was found increased thereafter in diabetic group. It suggested a more pronounced expression compared with age-matched nondiabetic controls. Conversely, galectin-3 was expressed in tubuli from both nondiabetic and diabetic rats, with an increased staining in diabetic versus nondiabetic animals at all time points examined. An intimate relationship existed between the expression of galectin-3 in glomeruli from diabetic rats and early diabetic nephropathy. Conclusion： in hyperglycemia condition, the up-regulating galectin-3 may play an important role in the progression of diabetic nephropathy, which awaits further investigation.

关 键 词：糖尿病肾病 GALECTIN-3 免疫组化 

分 类 号：R587.1[医药卫生—内分泌]