罗格列酮对高糖刺激下肾小管上皮细胞整合素β_1表达的影响  

Effect of rosiglitazone on the expression of integrin β_1 in proximal tubular cell exposed to high glucose

作　　者：唐晓红[1] 黄颂敏[1] 谭世桥[2] 任敏[3] 

机构地区：[1]四川大学华西医院肾脏内科,四川成都610041 [2]四川大学华西第二医院 [3]四川省疾病控制中心

出　　处：《西部医学》2006年第2期134-136,共3页Medical Journal of West China

摘　　要：目的观察罗格列酮(R os ig litazone,RO S)对肾小管上皮细胞整合素水平的影响。方法将体外培养的大鼠近端肾小管上皮细胞分为对照组、正常血糖组(N)、高糖组(H)、正常培养液+罗格列酮组(RO S)、高糖+罗格列酮低浓度组和高糖+罗格列酮高浓度组六组,采用W estern B lot及RT-PCR了解不同浓度的罗格列酮(5μm o l/L、10μm o l/L)对高糖刺激(30mm o l/L)下整合素1β(In tegrin-beta1)蛋白质水平表达的影响。结果高糖刺激下,In tegrin1β(84710.79±1810.4)较对照组(23283.17±827.55)及正常葡萄糖组(47896.31±2014.63)明显增加(P<0.05),RO S组In tegrin1β(22609.49±1076.26)较高糖组降低,RO S 10μm o l/L组(38603.43±1409.30)的降低程度较RO S 5μm o l/L组(31327.16±1721.36)更明显。结论罗格列酮可明显抑制高糖刺激下肾小管上皮细胞In tegrin1β的表达,且有明显剂量依赖关系。ObjectiveTo evaluate the effect of rosiglitazone on the level of integrin β1 in proximal tubular cells. Methods The proximal tubular cells of rats cultured in vitro were divided into 6 groups ： control group, normal glucose group, high glucose group, high glucose with rosiglitazone of 5μmol/L group, high glucose with rosiglitazone of 10μmol/L group and single rosiglitazone of 10μmol/L group. The cells werecuhured for 48 b. Integrin β1 was detected by Western blot. Results The expression of integrin 61 was significantly increased in high glucose group （84710. 79± 1810. 44）as compared with control group （23283.17±827.55）and normal glucose group（47896.31±2014.63）, Integrin β1 in high （30 mmol） dose of glucose group with 5 or 10 mmol roslglitazone was 31327.16±1721.36 and 38603.43± 1409.30, respectlvely（both P〈0. 005）. Conclusion Rosiglitazone can strikingly inhibit the expression of integrin β1 in proximal tubular cells exposed to high glucose with a barked dose-dependent manner.

关 键 词：近端肾小管上皮细胞 高糖 罗格列酮 整合素Β1 

分 类 号：R857.1[医药卫生—航空、航天与航海医学] R692[医药卫生—临床医学]