小麦T型细胞质雄性不育系和保持系蛋白质的比较研究  

Comparative Studies on the Constitution of Protein between T-type Cytoplasmic Male Sterile Wheat and Its Maintainer

作　　者：滕晓月[1] 陈雪晖 

机构地区：[1]北京农业大学生物学院,北京100094

出　　处：《作物学报》1996年第3期264-270,共7页Acta Agronomica Sinica

基　　金：北京农业大学植物生理生化开放实验室资助项目

摘　　要：以小麦T型雄性不育系及其保持系种子为材料,用双向电泳结合高灵敏度银染技术对其胚乳醇溶蛋白进行分析,发现两系之间有明显差异.不育系14A有两个特异蛋白,分子量分别为36kD和46kD,而缺少45kD的多肽;不育系11A有24kD的特异蛋白,且30kD及28kD的两种多肽,其浓度较保持系明显增加.又用梯度SDS-PAGE方法比较了不同发育时期(苗期、开花期、种子)可溶性蛋白的组分,发现两系之间在种子和花药中有明显差异,而在苗期叶片未发现显著差异,表明T型细胞质雄性不育基因的表达具有时空性.本文还对非平衡pH电泳的应用进行了讨论.Differences between male-sterile and maintainer lines of T. timopheevi wheat have been found in the protein constitution of seeds using silver-stained two-dimensional gel elec-trophoresis.Male-sterile line, 14A, has a 36 kD and a 46 kD polypeptides and no appearance of a 45 kD polypeptide which exists in its maintainer line, 14 B. The male-sterile line of 11A presents a 24 kD polypeptide which is absent in 11B. The content of 30 kD and 28 kD proteins in 11A is much higher than that in its maintainer line, 11 B.The soluble proteins of different stages (leaf, anther, seed) have been compared with gradient SDS polyacrylamide gel electrophoresis and the differences have been found in anthers and seeds.

关 键 词：小麦 细胞质雄性不育 特异蛋白 梯度SDS-PAGE 

分 类 号：S512.101[农业科学—作物学]