雷公藤单体T_4对类风湿关节炎患者周围血单个核细胞及滑膜细胞产生肿瘤坏死因子的影响  

The Effects of a Single Active Ingredient(T4)of Tripterygium Wilfordii Hook on the Production of Tumor Necrosis Factor by the Peripheral Blood Mononuclear Cells and Syuovium Cells of Rheumatoid Arthritis Patients

作　　者：曾学军[1] 张乃峥[1] 

机构地区：[1]中国医学科学院中国协和医科大学协和医院

出　　处：《中国医学科学院学报》1996年第2期138-142,共5页Acta Academiae Medicinae Sinicae

摘　　要：观察了经雷公藤单体Ｔ_４处理２ｈ后对正常人和类风湿关节炎（ＲＡ）患者外周血单个核细胞（ＰＢＭＣ）及ＲＡ患者消化的滑膜单个细胞（ＲＡ－ＤＳＳＣ）分泌ＴＮＦ的影响。在３５ｎｇ／ｍｌ浓度下，正常人ＰＢＭＣ（ｎ＝１０）及ＲＡ－ＰＢＭＣ（ｎ＝６）Ｔ_４处理组产生ＴＮＦ活性水平明显低于对照组（Ｐ＜０．００１）。延长Ｔ_４作用时间至６ｈ，ＲＡ－ＰＢＭＣＴ_４处理组ＴＮＦ活性水平下降更为明显（Ｐ＜０．００１），表明Ｔ_４可抑制ＰＢＭＣ体外产生ＴＮＦ活性水平，其对ＲＡ－ＰＢＭＣ作用不同时间的抑制率分别为２５．５４％及４０．５１％（Ｐ＜０．０１），说明Ｔ_４抑制作用可能与作用时间有关。同时Ｔ_４亦可抑制ＲＡ－ＤＳＳＣ体外产生ＴＮＦ活性水平（Ｐ＜０．０１），提示Ｔ_４有望成为一新的植物性治疗ＲＡ药物。Encouraged by good therapeutic results of the semipurmd preparations of TripterygiumWilfordii Hook(TWH)(code name T Ⅱ)a single active ingredient of TWH with the codename of T4 was obtained.The in vitro effects of T4 on the bioactivity of TNF produced byPBMC of normal subjects(n= 10)and RA patients(n=6)or the RA digested synovium sin-gle cells(DSSC)were studied.The results showed that under pretreatment of PBMC ofhealthy persons with T4(35ng/ml) for two hours,TNF were reduced to 36.13±1.75%incomparison with the control of 63.33 ± 2.51%(P<0.001),with an inhibition rate of40.31%.Similarly,the inhibition rate for the PBMC of RA patients(P<0.001),25.54%.The fact that coculture of PBMC and T4 for 6 hours instead of treatment with T4for 2 hours caused an inhibition rate of 40.51%(P<0.01),might indicate that time of in-teraction was also a factor of importance.T4 also reduced TNF secretion by RA-DSSC from72.1±6.1%to 51.6±2.0%with an inhibition rate of 28.4%.The aformentioned indicatesthat T4 appears to possess suppressive actions on production of TNF by PBMC and DSSC ofRA patients.Therefore,it is possible that T4 may be used clinically for RA patients.

关 键 词：雷公藤单体 类风湿性关节炎 肿瘤坏死因子 

分 类 号：R286.11[医药卫生—中药学] R593.22[医药卫生—中医学]