BL21（DE_3）／PET－11做为人IL－2表达系统的研究  

The Study of BL21(DE_3)/PET-11 as an Expression System of Human IL-2

作　　者：公茂凯[1] 章静波[1] 刘德培[1] 张世馥[1] 刘丕旭 董敏 

机构地区：[1]中国医学科学院,中国协和医科大学北京基础医学研究所

出　　处：《中国医学科学院学报》1996年第1期60-65,共6页Acta Academiae Medicinae Sinicae

摘　　要：ＢＬ２１（ＤＥ_３）／ＰＥＴ－１１做为人ＩＬ－２表达系统的研究公茂凯，章静波，刘德培，张世馥刘丕旭，董敏（中国医学科学院中国协和医科大学北京基础医学研究所，北京１００００５）关键词白细胞介素２，ＢＬ２１（ＤＥ_３），聚合酶链反应中图号Ｒ３９２。１２白细...Abstract The coding region of hlL-2 cDNA is specifically amplified by PCR.ANdeⅠsite is intro-duced into its5′-end and its3′-non coding region is partly deleted.After filling-in by Klenowenzyme,the DNA fragment is cloned into Sma Ⅰsite of PUCl 8.Eight white colonies arescreened and one(C1)contains NdeⅠ site.DNA sequencing shows that non-specific muta-tion has not been found in its coding region.The DNA fragment digested by BamH Ⅰ,Nde Ⅰ of C1 plasmid is recovered and subcloned into PET-11 and transfered into BL2l(DE3).After IPTG induction,the Bacteria lvsate is run directly on SDS-PAGE. Westernblot test shows a specific rhlL-2 McAb binding band.The molecular weight is about l7 000.Laser scanning indicates that the absorption area of IL-2 is about 5%of the total area.

关 键 词：白细胞介素2 BL21(DE3) 聚合酶链反应 

分 类 号：R392.12[医药卫生—免疫学]