人血小板密度亚群在聚集反应,ATP释放及细胞内游离钙浓度的异质性(英文)  

Heterogeneity of human platelet density subpopulations in aggregation secretion of ATP and cytosolic-free calcium concentration

作　　者：李柏岩[1] 何树庄[1] 李文汉[1] 白颖[2] 

机构地区：[1]哈尔滨医科大学药理教研室 [2]哈尔滨市第一医院神经内科

出　　处：《中国药理学报》1996年第2期152-155,共4页Acta Pharmacologica Sinica

摘　　要：目的:研究人血小板密度亚群在聚集和释放反应及细胞内钙浓度的异质性。方法:Percoll间断梯度离心法。结果:正常人血小板分为高(HD),中(ID)和低(LD)密度三个亚群。血小板的大小随密度的降低而减小(r=0.978,P<0.01)。HD对凝血酶(Thr),ADP和血清素(5-HT)诱导的聚集反应明显强于LD(P<0.01),ATP释放反应,除5-HT无ATP释放外,与聚集反应的结果一致。尽管细胞内游离钙([Ca^(2+)]_i的静息水平各亚群相同,但Thr,ADP和5-HT的[Ca^(2+)]_i动员仍以HD最为明显。结论:人HD血小板的功能及活性均高于LD血小板。AIM: To investigate thrombin (500 U·L-1)-, ADP (0. 1 - 30 μmol · L-1)-, and 5-hydrox-ytryptamine (5-HT, 3 μmol · L )-induced aggregation, secretion of ATP and cytosolic-free calcium mobilization in density subpopulations of human washed platelets. METHODS: Using Percoll discontinuous gradient. RESULTS: The human platelets were separated into high density (HD), intermediate density (ID), and low density (LD) subpopulations, and their sizes were diminished with decreasing density ( r = 0.978, P < 0.01). The magnitude of aggregations by thrombin, ADP, and 5-HT was more significant in HD platelets than that in LD platelets (P<0.01). The amount of secretion of ATP induced by thrombin and ADP in HD platelets was also much higher than that in LD platelets (P<0.01), except for 5-HT which did not cause the ensuring release reaction in any sub-population of human platelets. Thrombin (1500 U ·L-1)-, ADP (μmol·L-1)-, and 5-HT (3 μmol · L-1)-induced cytosolic-free calcium mobilization was evaluated as well. Results showed that the resting level of cytosolic-free calcium concentration ([Ca2+ ];) was the same in all subpopulations, about 80 - 90 nmol · L-1 . However, the level of [Ca2+ ]; mobilization was entirely different, heightened with increasing density. CONCLUSION: The function of HD platelets was much stronger and more active than that of LD platelets in human.

关 键 词：血小板聚集 钙 ATP 密度亚群 

分 类 号：R331.143[医药卫生—人体生理学]