不同甘油原料发酵生产1,3-丙二醇的研究  

Study on Microbial Production of 1,3-Propanediol with Different Raw Glycerol

作　　者：徐友海[1] 刘海军[1] 赵晶[1] 修志龙[1] 

机构地区：[1]大连理工大学生物科学与工程系

出　　处：《精细与专用化学品》2006年第5期14-17,共4页Fine and Specialty Chemicals

基　　金："十五"国家科技攻关项目(2001BA708B01-04);"973"计划项目(2003CB716000)

摘　　要：对克雷伯氏菌在厌氧条件下以甘油为原料发酵生产1,3丙-二醇进行了研究,比较了不同甘油对菌体生长和产物生成的影响。结果表明,采用批式流加发酵的方式,以纯甘油作为原料时,发酵液中1,3丙-二醇的质量浓度最高达到72.15g/L,从甘油到1,3丙-二醇的物质的量转化率为63.32%,生产强度达2.67g/(L.h);以粗发酵甘油作为原料时,发酵液中1,3丙-二醇的质量浓度最高为56.72g/L,物质的量转化率53.14%,生产强度2.36g/(L.h);而低纯度皂化甘油对菌体生长有较强的抑制作用。成本分析表明粗发酵甘油更具有工业化应用前景。The microbial conversion of glycerol into 1,3-propanediol （1,3-PD） by Klebsiella pneumoniae under anaerobic conditions was studied. The effects of different raw glycerol on the microbial growth and the product formation were investigated by fed,batch fermentations. The experimental results showed that the final concentration, molar yield and productivity of 1,3-PD were 72.15g/L,63.32% , and 2.67g/（ L. h） respectively by using pure glycerol. And the target parameters were 56.72g/L, 53. 14%, and 2.36g/（ L. h） respectively by using crude fermentative glycerol at the same fed-batch fermentation conditions. But the microbial growth was strongly inhibited by the untreated saponified glycerol. The cost analysis demonstrated that the crude fermentative glycerol could be used for the microbial production of 1,3-propanediol with more commercial prospects potentially.

关 键 词：1 3-丙二醇 克雷伯氏菌 厌氧发酵 甘油 

分 类 号：TQ223.162[化学工程—有机化工]