芦笋丛生芽增殖培养技术  

Study on Enrichment Culture of Asparagus officinalis Cespitose Buds

作　　者：林宗铿[1] 罗金水[1] 蔡坤秀[1] 陈振东[1] 高海筹[1] 黄德贵[1] 

机构地区：[1]福建省热带作物科学研究所,福建漳州363001

出　　处：《亚热带植物科学》2006年第1期29-32,共4页Subtropical Plant Science

基　　金：福建省科技厅重点项目(2002N033)

摘　　要：本试验以芦笋新品种“津宁”为材料，探讨植物生长调节剂对不同外植体产生丛生芽的效应。试验结果表明，NAA、6-BA和KT三者配合使用有利于丛生芽的增殖：以茎尖为增殖外植体时，培养基用MS＋NAA0．1me/L＋6-BA0．2-0．3mg／L＋KT0．1mg／L，30d时平均每瓶可以产生20．5个丛生芽；以茎中段为外植体时，培养基用Ms＋NAA0．1mg／L＋6-BA0．3-0．4mg／L＋KT0．1mg／L，30d时平均每瓶可产生33．5个丛生芽；以茎基为外植体时，培养基用MS＋NAA0．1mg／L＋6-BA0．3-0．5mg／L＋KT0．1mg／L，30d时平均每瓶可以产生43．1个丛生芽。进行试管苗工厂化生产时，增殖外植体以茎基为最佳。Effects of plant growth regulators on multiplication of cespitose buds from Asparagus officinalis new species ＇Jinling＇ were studied. The result indicated that the admixture of NAA, 6-BA and KT gave good result for multiplication of cespitose buds. Taking the stem apex as explant, after 30d, there were 26.3 cespitose buds per bottle when cultured in MS ＋ NAA 0.1mg/L ＋ 6-BA 0.2- 0.3mg/L ＋ KT 0.1mg/L; taking the mid stem as explant, after 30d, there were 33.5 buds per bottle when cultured in MS ＋ NAA 0. I mg/L ＋ 6-BA 0.3 -0.4mg/L ＋ KT 0.1mg/L; taking the stem base as explant, there were 43.1 buds per bottle when cultured in MS ＋ NAA 0. lmg/L ＋ 6-BA 0.3-0.5mg/L ＋ KT 0.1mg/L. The stem base was the optimum explant for rapid propagated.

关 键 词：芦笋 离体培养 丛生芽 增殖 

分 类 号：Q943.1[生物学—植物学] S644.6[农业科学—蔬菜学]