青菜花粉管通道法导入外源DNA的研究初报  

Preliminary study of the introduction of foreign DNAs into cabbage(Brassica chinensis L.) via pollen-tube pathway

作　　者：王光清[1] 王蕴珠[1] 胡容霞[1] 杨金水[1] 葛扣麟[1] 李美珍 陈根法[1] 郑振钧 王吟雪 王垂丁 

机构地区：[1]复旦大学遗传学研究所,闸北区彭浦乡

出　　处：《复旦学报（自然科学版）》1996年第2期127-131,共5页Journal of Fudan University：Natural Science

基　　金：上海市科学技术发展基金

摘　　要：采用花粉管通道法，将青菜株系７６Ｆ５、１４号大白菜、紫阳等三个品种的总ＤＮＡ及质粒ｐＡｃｔｌ－ＤＤＮＡ导入青菜６０５品系，收获青菜种子．种子发芽后分别提取约两周苗龄的小苗总ＤＮＡ，并经ＥｃｏＲＩ酶切，以ｇｕｓ基因片段为探针做分子杂交．证实了外源ｇｕｓ基因已整合到青菜６０５品系中；田间观察还获得了一株与供体大白菜花形相似的变异株．otal DNAs of Brassica chinensis L. strain 76F5, Brassica pekinensis L.,Brassica oleracea var rubra L. and plasmid pActl-D were introduced into Brassica chinensis L. variety 605 via pollen-tube pathway. Seeds from the foreign DNA-treated plants were harvested and allowed to germinate. Hybridization of the gus gene fragment probe to products of EcoR I-digested total DNAs from various plants were carried out and hybridization bands were detected in the total DNAs of progenies from foreign DNA-treated plants. A variant similar to the donor Brassica pekinensis L. was obtained among progenies.

关 键 词：青菜 分子育种 花粉管通道 外源DNA导入 育种 

分 类 号：S636.03[农业科学—蔬菜学]