水稻广陆矮4号基因文库的构建及U2snRNA基因的筛选  

Construction of rice genomic library and screening of U2snRNA genes

作　　者：王喜萍[1] 詹树萱[1] 刘晓斐 曹凯鸣[1] 

机构地区：[1]复旦大学生物化学系

出　　处：《复旦学报（自然科学版）》1996年第2期145-149,共5页Journal of Fudan University：Natural Science

基　　金：国家科委863高科技项目

摘　　要：水稻广陆矮４号黄化苗总ＤＮＡ经Ｓａｕ３Ａ部分酶切，回收１２～２３ｋｂ片段，经ＣＩＰ脱磷后克隆于ＥＭＢＬ３载体，建立了水稻基因文库．以拟南芥的Ｕ２．２ｓｎＲＮＡ基因为探针，从基因文库中筛选到１３个阳性克隆，经不同酶切鉴定，初步判断有８种不同的克隆，对其中一个克隆ＦＤＲＧＵ２－３的重组ＤＮＡ构建了物理图谱，Ｕ２ｓｎＲＮＡ的一个基因已经定位在该克隆中１．５ｋｂ的Ｓａｉｌ－ＢａｍＨⅠ酶切片段上．fter San3A partial digestion of total DNA from etiolated seedling of rice(Oriza Sativa,indica variety. Guang Lu Ai 4),the 12￣ 23 kb DNA fragments were prepared by centrifugation in 10% ￣40% continuous sucrose density gradient and dephosphorized with CIP. The treated DNA fragments were ligated to arms of baeteriophage EMBL 3 vectors and a rice genomic library was constructed by in vitro packaging. From this library, 13 positive clones containing U2snRNA genes have been screened by in situ hybridization of bacteriophage λ plaques. The digestion pattern of recombinant DNA with several restriction endonucleases showed that there were 8 different clones found in 13 positive ones. One of the rice U2snRNA genes was located in a 1. 5 kb Sal I-BamH I fragment of the FDRGU2-3 clone.

关 键 词：黄化苗 基因文库 U2snRNA基因 水稻 

分 类 号：S511.01[农业科学—作物学]