胶体金免疫层析法快速检测鼠疫耶尔森菌F1抗体  

Rapid detection of the fraction-1(F1) antibody against Yersinia pestis with colloidal gold-immunochromatography

作　　者：胡孔新[1] 王静[1] 周蕾[2] 闫中强[2] 李伟[1] 姚李四[1] 牛春莉[1] 李瑾[1] 马颖[1] 陈维娜[1] 

机构地区：[1]中国检验检疫科学研究院,北京100025 [2]军事医学科学院微生物流行病研究所

出　　处：《中国人兽共患病学报》2006年第3期198-201,共4页Chinese Journal of Zoonoses

基　　金：国家质量监督检验检疫总局反生物恐怖重大专项资助(编号:研2004-5)

摘　　要：目的建立检测鼠疫耶尔森菌F1抗体的胶体金标记免疫层析方法。方法胶体金标记纯化鼠疫F1抗原,双抗原夹心法原理制成免疫层析检测试纸条,并对兔、羊、人和黄鼠血清进行检测评价。结果用80份不同动物种属的阴性血清进行检测,未出现非特异性反应,对5份恢复期病人血清、13份免疫兔血清和2份免疫羊血清检测也取得阳性结果,对于8份I HA临界阳性(滴度1∶20)的达乌尔黄鼠血清检出阳性6份。结论建立了可以用于现场快速检测鼠疫F1抗体的胶体金免疫层析方法。A method for rapid detection of the fraction 1 （F-1 ） antibody against Yersinia pestis was developed, in which the purified F-1 antigen was coupled with colloidal gold to be used for the detection of F-1 antibody by means of the double antigen sand- wich. The sensitivity, specificity and stability of this method of detection were evaluated by the testing with different sera of animals （rabbits, goats, humans and rattus）. A 100% specificity was demonstrated, when 8 IHA-negative rabbit sera, 30 negative Sper- mophilus dauricus sera observed in Western blot analysis, 4 normal goat sera and 38 normal human sera were assayed with this method. When 8 IHA positive S. dauricus sera （titer： 1：20） were assayed, 6 positive results were obtained. In addition, positive resuits were also obtained from 13 immunized rabbit sera, 2 immunized goat sera and 5 human sera. It is concluded a simple and rapid colloidal gold-immunochromatography assay for the detection of F-1 antibody against Y. pestis is established in the present study.

关 键 词：鼠疫菌 F1抗原 胶体金 免疫层析法 

分 类 号：R378.6[医药卫生—病原生物学]