日本血吸虫FKBP12基因的克隆及其免疫组织定位  

Cloning and immunohisto-localization of the FKBP12 gene of Schistosoma japonicum

作　　者：李孜[1,2] 余新炳[3] 吴忠道[3] 胡旭初[3] 

机构地区：[1]广州医学院病原生物学教研室 [2]中山大学基础医学院寄生虫学教研室,广州510180 [3]中山大学基础医学院寄生虫学教研室

出　　处：《中国人兽共患病学报》2006年第3期236-239,262,共5页Chinese Journal of Zoonoses

基　　金：国家教育部博士点基金(200045);广东"211工程"重点建设项目(98169)

摘　　要：目的克隆日本血吸虫(Sj)FKBP12基因全长cDNA,并进行免疫组织定位。方法从成虫RNA中RT-PCR扩增SjFKBP12基因完整的编码阅读框后,将其分别克隆入pGEX-4T-1和pEGFP-N1载体中;DNA免疫后制备抗血清,间接免疫荧光法对FKBP12进行组织定位。结果将SjFKBP12基因成功克隆入pGEX-4T-1和pEGFP-N1载体中;FKBP12定位于Sj成虫的被膜上。结论成功克隆了SjFKBP12基因,并对FKBP12蛋白在Sj成虫中进行组织定位,为将其进行疫苗等研究打下了基础。The cDNA of the FKBP12 gene of Schistosoma japonicum （ Sj ） was cloned and its immunohisto-loacalization was investigated in the present study, in which the full length of this gene was obtained from the adult worm RNA of S. japonicum by RT-PCR, and then was cloned into vectors of pGEX-4T-1 and pEGFP-N1, and indirect immunofluorescence assay was used to find its histo-localization in various tissues. It was demonstrated that the FKBP12 gene eDNA sequence could be cloned successfully into vectors pGEX-4T-1 and pEGFP-N1, and localized on the tugment of S. japonicum. The result of this investigation provides basis for the further studies on vaccine development.

关 键 词：日本血吸虫 中国大陆株 FKBP12 克隆 免疫定位 

分 类 号：R383.24[医药卫生—医学寄生虫学]