附红细胞体病瑞氏、姬姆萨、吖啶橙染色法及PCR检测法的比较研究  

Comparison of staining method with Wright, Giemsa and acridine stains with PCR assay for the detection of Eperythrozoon

作　　者：郑丽艳[1] 张衍俊 常维山[1] 

机构地区：[1]山东农业大学动物科技学院,泰安271018 [2]山东省嘉祥县畜牧局

出　　处：《中国人兽共患病学报》2006年第3期243-245,共3页Chinese Journal of Zoonoses

摘　　要：目的比较瑞氏、姬姆萨、吖啶橙染色法以及PCR法对附红细胞体的检出率,探索比较可靠的检测附红细胞体的方法。方法以瑞氏、姬姆萨、吖啶橙染色法以及PCR法检测附红细胞体病的方法的建立为基础,对20例病例用四种方法进行检测,并比较其检出率。结果瑞氏、姬姆萨、吖啶橙染色法以及PCR法的检出率分别为20%、30%、85%、80%,PCR与三种染色法的符合率分别为35%、50%、95%。结论吖啶橙及PCR法优于瑞氏及姬姆萨氏染色法。The detection rates of the staining methods with Wright, Giemsa and acridine stains were compared in order to search for a reliable method to detect the presence of Eperythrozoon. It was found that the positive rates of these 4 methods of detection in 20 blood specimens of patients with Eperythrozoon disease were 20 %, 30 %, 85 % and 80 % respectively, and the consistency rates of PCR assay with the 3 staining methods were 35 %, 50 % and 95 % respectively, indicating that the acridine staining method and PCR assay were better than Wright and Giemsa staining methods for the detection of Eperythrozoon in clinical specimens.

关 键 词：附红细胞体 瑞氏 姬姆萨 吖啶橙 PCR 

分 类 号：Q934.3[生物学—微生物学]