卫氏并殖吸虫致病品系PCR-RAPD分子标记的初步研究  

PCR-based random amplified polymorphic DNA technique used for the genetic variations of pathogenic strains of Paragonimus westermani

作　　者：钱宝珍[1] 沈琦 

机构地区：[1]浙江省医学科学院生物工程研究所,杭州310013 [2]浙江省中医学院

出　　处：《中国人兽共患病学报》2006年第3期249-251,共3页Chinese Journal of Zoonoses

基　　金：国家科技部中国-泰国长期合作项目"肺吸虫病的研究"(No.17-603J);浙江省科技厅项目(2005F13023)

摘　　要：目的探讨卫氏并殖吸虫致病品系PCR-RAPD分子标记。方法应用随机扩增多态DNA(PCR-RAPD)技术,对浙江宁海小汀、宁海西溪、遂昌、临安等地卫氏并殖吸虫囊蚴进行遗传变异研究。结果通过8条寡核苷酸随机引物扩增,B17-1600bp为浙江宁海小汀肺吸虫种群特异性DNA片段,A9-680bp为浙江宁海西溪,遂昌、临安三地肺吸虫种群共有特异性DNA片段。结论结果提示B17-1600bp,A9-680bp可作为卫氏并殖吸虫不同致病品系的分子标记。The PCR-based random amplified polymorphic DNA （PCR-RAPD） technique using 8 deeamer oligonueleotide primers was employed to detect the genetic variations of pathogenic strains of Paragonimus westermani isolated in Zhejiang province. The result showed that the B17-1600 bp fragment was the strain-specific DNA fragment of population of P. vaeatermani in Xiaoting area of Ninghai, while the A9-680 bp fragment was the specific DNA fragment of population in Xixi area of Ninghai, Shuiehang and Lin＇ an. This result indicates that both the B17-1600 bp and A9-680 bp fragments can be used as the molecular markers for identification of pathogenic P. westermani strains isolated in Zhejiang province.

关 键 词：卫氏并殖吸虫 RAPD 致病性 

分 类 号：R383.2[医药卫生—医学寄生虫学]