压电石英晶体传感器检测血浆凝血酶时间的实验研究  

Experimental study of piezoelectric quartz crystal sensor for detecting plasma thrombin time

作　　者：张波[1] 府伟灵[1] 张雪[1] 杨新[1] 陈庆海[1] 俞凡[2] 

机构地区：[1]第三军医大学西南医院检验科,重庆400038 [2]中国电子科技集团公司第二十六研究所,重庆400060

出　　处：《重庆医学》2006年第5期415-417,共3页Chongqing medicine

基　　金：国家"863"计划资助项目(2002AARZ2023);国家自然科学基金资助项目(20405016);国家科技部九五科学仪器攻关项目<科学仪器和实验条件研究与开发>(96-A23-04-04)

摘　　要：目的建立一种检测血浆凝血酶时间(TT)的压电石英晶体传感器。方法采用AT切向、基频10MHz的银膜石英晶体作为压电元件;探讨反应体系密度和黏度与晶体振荡频率变化的关系,观察血浆凝集过程中晶体振荡频率的变化规律,确定判断血浆凝集反应起点和终点的方法。结果(1)反应体系加入凝血酶试剂启动凝血反应后,晶体振荡频率呈阶梯状快速下降,接着频率瞬时上升,然后再缓慢降低达到平稳,频率阶梯下降的起点为血浆凝集反应的起始点,频率从快速上升变为下降的转折点为血浆凝集的终点,计算两点之间的时间即为血浆凝血酶时间。(2)该方法的检测结果与STAGO血凝仪磁珠检测法的相关性好(γ=0.964),批内CV=2.26%,批间CV=7.53%。结论压电石英晶体传感器检测血浆凝血酶时间,血浆凝集反应起点和终点确定方便、可靠。且操作简单、快速、成本低廉,检测结果准确,是一种敏感性高、重复性好的血浆凝血酶时间检测新方法,可用于临床检测。Objective To establish a piezoelectric quartz crystal sensor for determination of the plasma thrombin time（TT）. Methods The quartz crystal was 10MHz quartz AT-cut with silver coated electrodes. The oscillating frequency changes were observed in the course of the coagulation reaction of plasma sample, the starting point and the end point of the coagulation reaction were showed by the frequency change tendency. Results （1） After the thrombin was added into the reaction system, oscillation frequency of the crystal was decreased as a ladder, then rose abruptly and descended quickly to balance. The start of the ladder-like frequency decrease was regarded as the start point of coagulation reaction, the turning point of the frequency from rise to decrease as the end point of the reaction. The time between the start point and the end was calculated as TT. （2）The result of statistic analysis showed there was a significant correlation between the results from our method and STAGO method （γ= 0. 964）, the variances within and between batches were 2.26 % and 7.53 % respectively. Conclusion Piezoelectric quartz crystal sensor method for determination of the plasma thrombin time is simple, rapid, specific, accurate, cheap, and it might be a valuable method for detection of TT in clinical laboratory.

关 键 词：压电 石英晶体 传感器 凝血酶时间 压电石英晶体传感器 

分 类 号：R446.11[医药卫生—诊断学]