胞外黏质物在心血管材料表面细菌生物膜形成中的作用  

Effect of Extracellular Slime Substance on Bacterial Biofilm Formation on Surface of Biomaterials

作　　者：黄云超[1] 张良[1] 林兴[1] 杨达宽[2] 李高峰[1] 王曦[2] 

机构地区：[1]昆明医学院第三附属医院,云南昆明650118 [2]昆明医学院第二附属医院,云南昆明650101

出　　处：《中华医院感染学杂志》2006年第3期292-295,共4页Chinese Journal of Nosocomiology

基　　金：国家自然科学基金(30360103);云南省科技厅国际合作计划项目基金(2002GH09)

摘　　要：目的 通过体外试验观察、预处理的涤纶材料表面细菌生物膜（BF）形成的影响，为临床以生物材料为中心的感染（BCI）提供有益的思路。方法 用生物膜形成阳性的表皮葡萄球菌RP62A和生物膜形成阴性的表皮葡萄球菌M7，在普通液体培养基及其调整浓度后的培养基中，对以血浆预处理和未处理的涤纶材料进行体外动态生物膜形成试验，用激光共聚焦显微镜（CLSM）测定生物膜厚度，用环境扫描电子显微镜（SEM）直接观察涤纶材料上细菌生物膜形成的情况。结果 RP62A与M7在两组材料表面形成BF差异有显著性（P〈0．05）；RP62A在不同处理材料表面BF形成有显著区别（P〈0．05）；培养基调整组与培养基正常组生物膜厚度比较差异有显著性（P〈0．05）。结论 细菌生物膜在生物材料表面形成是动态的过程，其高峰期在24～30h；血浆预处理生物材料和培养基调整组能增加胞外黏质物（ESS）的分泌和促进细菌生物膜形成；ESS在生物材料表面细菌生物膜形成中起重要作用，阻断ESS产生及其作用必将为临床治疗以生物材料为中心的感染提供帮助。OBJECTIVE To observe the influence of Staphylococcus epidermidis （SE） existing in different liquid culture on bacterial biofilm formation on Dacron and to provide beneficial way for the purpose of the prophylaxis of bacterial centered infection （BCI）. METHODS One group used normal liquid culture, the others used culture with the concentration of 2% NaCl and 3% glucose. Some Dacron strips were preincubated for 120 minutes at 37℃ with plasma and others did not, then taken the experiment of bacterial biofilm formation by SE and by its adhesion-positive, accumulation-negative mutant M7. The density of bacterial biofilm associated with Dacron strips was measured by confocal laser scanning microscope （CLSM）. At the same time, the scanning electron mi croscope （SEM） was used to observe the configuration of BF on the surface of Dacron segments. RESULTS There was a significant difference of density of BF on Dacron strips between RP62A and M7 （P〈0.05） ; plasma-preincu- bated Dacron strips increased the density of BF obviously when compared with the none-treated ones in RP62A group （P〈0.05） ; the study group of liquid culture had more density of BF than control one （P〈0.05） ; BF formation pear phase was 24-30 hours surveyed from SEM and CLSM. CONCLUSIONS BF formation on the surface of biomaterials is a dynamic process whose peak time is 24-30 hours. Both plasma-preincubated Dacron and adjusted liquid culture can stimulate the bacteria to secrete extracellular slime substance （ESS） and promote the formation of BF. Our experiment indicated that ESS plays a great role in BF formation on the surface of biomaterials.

关 键 词：胞外黏质物 生物材料 细菌生物膜 

分 类 号：R543[医药卫生—心血管疾病]