妊高征大鼠颈交感神经干离断对胎鼠脑的保护作用  

Protective effect of transection of cervical sympathetic trunk on fetal brain in rats with pregnancy-induced hypertension

作　　者：张丽红[1] 马铃[1] 崔健君[1] 

机构地区：[1]中国医科大学附属第二医院麻醉科,沈阳市110003

出　　处：《中华麻醉学杂志》2006年第1期75-77,共3页Chinese Journal of Anesthesiology

基　　金：辽宁省教委资助课题(20121270)

摘　　要：目的探讨妊高征(PIH)大鼠颈交感神经干离断(TCST)对胎鼠脑的保护作用。方法妊娠 Wistar 大鼠32只,随机分为4组,每组8只,对照组(A 组)、PIH 组(B 组)、TCST 组(C 组)和假手术组(D 组)。B 组自孕14d 开始皮下注射 L-硝基精氨酸甲酯(L-NAME)12.5mg/100g,每天平均分两次注射,至孕20d,A 组给予等量生理盐水；C 组孕14d 行右 TCST,余同 B 组；D 组孕14d 行右颈交感神经干分离,但不离断,余同 B 组。各组于孕21d 剖宫取仔,观察胎鼠脑组织超微结构,测定脑组织 ATP、ADP、AMP 的含量、腺苷酸池(ATP+ADP+AMP)及神经元细胞膜 Na^+-K^+-ATP 酶及 Ca^(2+)-ATP 酶活性。结果 A、C 组脑组织超微结构基本正常,B、D 组脑组织超微结构严重受损。与 A 组比较,B、D 组脑组织 ATP、ADP、AMP 的含量、腺苷酸池及神经元细胞膜 Na^+-K^+-ATP 酶及 Ca^(2+)-ATP 酶的活性均降低；与 B 组比较,C 组脑组织 ATP、ADP、AMP 的含量、腺苷酸池及神经元细胞膜 Na^+-K^+-ATP 酶及 Ca^(2+)-ATP 酶的活性均升高(P＜0.01)。结论 PIH 大鼠 TCST 对胎鼠脑有一定的保护作用,可能与改善脑细胞的能量代谢及相关的离子泵功能有关。Objective To investigate the protective effect of transection of cervical sympathetic trunk （TCST） on the fetal brain in rats with pregnancy-induced hypertension （PIH）.Methods Thirty-two pregnant Wistar rats were randomly divided into 4 groups （ n = 8 each） ： A control group; B PIH group; C TCST ＋ PIH group and D sham operation ＋ PIH group. PIH was produced by L-NAME 12.5 mg· 100g^-1 given subcutaneously from 14^th-20^th day of gestation. In group A normal saline was given instead of L-NAME. In group C TCST was performed on the 14^th day of gestation and L-NAME was injected asin group B. In group D the cervical sympathetic trunk was only exposed but not cut. Caesarean section was performed and fetus was taken on the 21^th day of gestation. The uhrastructure of fetal brain was examined. The content of ATP, ADP and AMP in the fetal brain and the Na^＋-K^＋-ATPase and Ca^2＋-ATPase activity in neuronal plasmalemma were determined. Results The uhrastructure of the fetal brain was almost normal in group A and C, but was seriously damaged in group B and D.The ATP and adenylic acid content in the brain tissue and the Na^＋ -K^＋ -ATPase and Ca^2＋ -ATPase activity in neuronal plasmalemma were significantly lower in group B and D than in group A and C. Conclusion TCST has protective effect on the fetal brain in rats with PIH by improving energy supply and enhancing pump function of neuron.

关 键 词：交感神经切除术 妊娠并发症 心血管 胚胎和胎儿发育 脑 妊高征 

分 类 号：R714.246[医药卫生—妇产科学] R651[医药卫生—临床医学]