硫唑嘌呤与牛血清白蛋白相互作用的热力学研究  

Spectrometric study on the interaction between Azathioprine and bovine serum albumin

作　　者：原华平[1] 关洪亮[1] 顾青[1] 潘祖亭[1] 

机构地区：[1]武汉大学化学与分子科学学院,武汉430072

出　　处：《分析试验室》2006年第3期81-85,共5页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金资助项目(20275028)

摘　　要：用荧光光谱法和紫外-可见光谱法研究了在模拟人体生理条件下,硫唑嘌呤和牛血清白蛋白(BSA)结合反应的特征,发现硫唑嘌呤对BSA有较强的荧光猝灭作用,且硫唑嘌呤的紫外吸收光谱和BSA的荧光光谱有一定程度的重叠,由此可得出其作用距离和结合过程的基本热力学参数。The binding characteristics of Azathioprine （AZP） and bovine serum albumin （BSA） were studied by means of fluorescence and absorption spectroscopy in aqueous solutions. The results showed that AZP had a powerful ability to quench the BSA fluorescence via non-radiation energy transfer mechanism. The fluorescence quenching data were analyzed according to Stem-Volmer equation and double-reciprocal equation, and the reaction distance and the thermodynamic parameters were obtained.

关 键 词：硫唑嘌呤 牛血清白蛋白 荧光光谱法 吸收光谱法 热力学 

分 类 号：O657.32[理学—分析化学]