高效液相色谱法与酶联免疫试剂盒测定海水中痕量氯霉素  

Determination of chloramphenicol in sea water by high performance liquid chromatography and ELISA

作　　者：胡莹莹[1] 李爱[1] 叶赛[1] 王菊英[1] 马德毅[1] 

机构地区：[1]国家海洋环境监测中心

出　　处：《分析试验室》2006年第3期112-114,共3页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金资助项目(40376035)

摘　　要：详细报道了海水样品中氯霉素的高效液相色谱检测方法及酶联免疫试剂盒检测方法。应用高效液相色谱法测定海水中氯霉素，方法的线性范围为5～50ng，检出限为0．064ng，回收率为96．2％-118．4％，相对标准偏差小于8．9％。酶联免疫试剂盒测定海水中的氯霉素背景值偏高，在加标浓度为10ng／mL时回收率为101．0％，相对标准偏差为8．32％。Chloramphenicol in sea water was determined by high performance liquid chromatography and ELISA. The linear range of the HPLC method is 5 - 50 ng, the detection limit is 1.28 ng/L. Recovery varies in the range of 96.2% - 118.4% with relative standard deviation of 8.9% . The detection limit of the ELISA method is 4 ng/mL, and the background value is high, when the spiked content is 10 ng/mL, the recovery is 101.0% with relative standard deviation of 8.32 %.

关 键 词：高效液相色谱 酶联免疫试剂盒 氯霉素 海水 

分 类 号：O657.7[理学—分析化学]