壳聚糖对预防大鼠腹膜粘连作用的研究  

Effects of Chitosan on Preventing Peritoneal Adhesion in Rats

作　　者：章志量[1] 周卸来[1] 

机构地区：[1]杭州师范学院临床医学院,杭州310018

出　　处：《中国药学杂志》2006年第5期343-346,共4页Chinese Pharmaceutical Journal

基　　金：国家自然科学基金资助项目(50173023)

摘　　要：目的探讨壳聚糖凝胶和壳聚糖/明胶共混膜对预防大鼠腹膜粘连的作用。方法实验分为壳聚糖凝胶组(A组)和壳聚糖/明胶共混膜组(B组)。A组分成:创伤致粘连组(A1组);滑石粉致粘连组(A2组);结扎血管致粘连组(A3组)。每组再随机分成对照组和实验组。实验组用壳聚糖凝胶均匀涂布于经处理的大鼠蚓突浆膜面。B组分成B1,B2,B3,B4 4组,在B2,B3,B4组大鼠的创面分别用纯壳聚糖膜、含10%,50%明胶的共混膜进行覆盖;B1组为对照组。术后2,4周,肉眼评定粘连程度和病理学检查。结果A组:①粘连评定:术后2,4周,A1和A3组中实验组的粘连程度均分别显著低于对照组,而A2组中的实验组和对照组间均无明显差异。②病理改变:A1和A3组以纤维组织增生为主。A2组有异物巨细胞反应及肉芽肿形成。B组:①膜降解速度:由快到慢依次为B4,B3和B2组;②术后2,4周:B1组的粘连程度均轻于其他组;病理组织学检查:实验各组局部以炎性细胞浸润及纤维组织增生为主,均见有异物巨细胞反应。结论壳聚糖凝胶对创伤及缺血所致的腹膜粘连有明显的预防作用;壳聚糖膜可加重腹膜粘连,而明胶的掺入可进一步促进粘连的形成。OBJECTIVE To investigate the effects of chitosan gel and chiston/gelatin mixed film on preventing peritoneal adhesion in rats, METHODS 240 rats were divided into jumpbogroup A（144 rats, with chitosan gel） and jumpbogroup B（96 rats, with chiston/gelatin mixed film）. Jumpbogroup A were further divided into 3 groups： the peritoneal adhesion were induced with injury（ group A1 ） ,Talc Powder （A2） and blood vessel Ligation（A3） respectively. Each group were randomly divided into control group and experimental group further. In the experimental group, chitosan gel were spreaded over the serosal surface of the treated procussus vermiformis.Jumpbegroup B were divided into 4 groups further： while the wound surface of procussus vermiformis in group B2, B3 and B4 were coverd with pure chitosan film,chiston film containing 10% gelatin and chiston film containing 50% gelatin respectively, no film was used in group B1 as control group,2 and 4 weeks after surgery, the peritoneal adhesion degree were evaluated with naked eye after celiotomy, and the adhesive blind-ending vermiform processes tissues were histopathologically observed. RESULTS In jumpbogroup A： ① peritoneal adhesion evaluation： 2 and 4 weeks after surgery, the experimental group in group A1 and A3 had milder adhesions than that in the control group. But in group A2, there was no significant difference between the control and experimental group, ②pathological change： group A1, A3 was mainly with fibroplasia, while massive foreignbody giant cell reaction（FBGCR） and granuloma fomation were observed in group A2. In jumpbogroup B：① degradation speed of film： group B3 had a faster degradation than group B2 but slower than group B4. ②2 and 4 weeks after surgery, adhesions in Group B1 was milder than any other experimtenal groups treated with various films;2 weeks after surgery, the adhesions in group B2 was significantly milder than that in group B3 and B4;4weeks after surgery, there were no significant difference of adhesions l

关 键 词：壳聚糖 壳聚糖/明胶共混膜 腹膜 粘连 

分 类 号：R965[医药卫生—药理学]