盐酸氨溴索和3,5二溴-邻氨基苯甲醛的液相色谱分析  

Analysis of Ambroxol Hydrochloride and 3,5-Dibromo-anth-o-animobenzaldehyde by Liquid Chromatography

作　　者：陈志斌[1] 蒋银土[2] 

机构地区：[1]金华职业技术学院,浙江金华321007 [2]浙江大学,浙江杭州310000

出　　处：《金华职业技术学院学报》2006年第1期76-77,85,共3页Journal of Jinhua Polytechnic

摘　　要：本文报道了用液相色谱法对盐酸氨溴索和3,5-二溴邻氨基苯甲醛同时进行检测分析。实验采用LC-10AD液相色谱仪,紫外检测。淋洗液为0.02mol/LNa2SO4加60%甲醇溶液(体积比),流速为1mL/min。在S/N=3时,盐酸氨溴索和3,5-二溴邻氨基苯甲醛的检测极限分别为0.05μg/mL、0.016μg/mL。盐酸氨溴索的保留时间、峰高和峰面积的变异系数分别为4.6061%、2.8984%、2.7333%,3,5-二溴邻氨基苯甲醛的保留时间、峰高和峰面积的变异系数分别为1.4649%、4.2684%、2.8002%。在线性范围5￣100μg/mL,盐酸氨溴索的峰高和峰面积的线性回归系数分别为0.9985、0.9990;在线性范围2￣40μg/mL,3,5-二溴邻氨基苯甲醛的峰高和峰面积的线性回归系数分别为0.9948、0.9939。本法简便、准确、重现性好。A liquid chromatography （LC） system with UV detector for the detection of ambroxol hydrochloride and 3,5-dibromo-anth-o-animobenzaldehyde was described. The elution contained 0.02mol/L Na2SO4 and 60% CH30H （1mL/min）. The RSD of ambroxol hydrochloride were 4.6061% for retention time, 2.8984% for height and 2.7333% for area. The RSD of 3,5-dibromo-anth-oanimobenzaldehyde were 1.4649% for retention time, 4.2684% for height and 2.8002% for area. The clibration graph was linear for 5-100μ g/mL of ambroxol hydrochloride with a detection limit of 0.05μg/mL; recoveries were 0.9985 for height, 0.9990 for area. The clibration graph was linear for 2-40μg/mL of 3,5-dibromo-anth-o-animobenzaldehyde with a detection limit of 0.016μg/mL; recoveries were 0.9948 for height,0.9939 for area. The method was simple, accurate and reproducible.

关 键 词：盐酸氨溴索 3 5-二溴邻氨基苯甲醛 液相色谱 紫外检测 

分 类 号：O657.72[理学—分析化学]