低密度脂蛋白对静脉内皮细胞抗凝功能的影响  

The Effects of Anticoagulant Function of Cultured Bovine Venous Endothelial Cells on the Low Density Lipoprotein

作　　者：经建中 周凤鑫[1] 黄桂秋[1] 葛志英 张健[1] 徐也鲁[1] 

机构地区：[1]上海第二医科大学病理生理学教研室,上海血液学研究所基础实验室,上海中医药大学生物化学教研室。

出　　处：《上海第二医科大学学报》1996年第2期84-87,共4页Acta Universitatis Medicinalis Secondae Shanghai

基　　金：国家自然科学基金;上海市高教基金的课题;并受与应洲基金

摘　　要：作者通过不同浓度的低密度脂蛋白（ＬＤＬ）与培养的新生小牛腔静脉内细胞（ＥＣ）孵育后，应用凝血酶、蛋白Ｃ和Ｓ－２２３８的发色底物显色法，检测单层ＥＣ膜上血栓调节蛋白（ＴＭ）辅助激活产生活化蛋白Ｃ（ＡＰＣ）的量，以此观察ＴＭ功能的变化。结果显示：ＬＤＬ孵育浓度≥２５μｇ／ｍｌ后，ＡＰＣ生成受抑；两者间呈负相关（ｒ＝－０．８７，ｎ＝２８６，Ｐ＜０．００１）；随着ＬＤＬ孵育时间延长，ＴＭ受抑加重，６ｈ孵育后受抑较２ｈ为著；动力学分析表明ＬＤＬ预处理使ＡＰＣ整个生成过程受抑；降低了ＡＰＣ生成率，早期尤甚，使生成率－反应时间曲线的曲率半径增大；ＡＰＣ生成反应速率出现前期减慢、后期加速的变化等。这些变化提示高脂可以通过抑制血管壁ＴＭ功能来影响机体局部抗凝功能。The changes of thrombomodulin (TM) activity were observed after cultured newborn bovine caval endothelial cells (EC) were incubated with variable concentrations of low density lipoprotein (LDL)in different incubation time by measurement of TM-dependent protein C activation on the surface of EC monolayers with thrombin and purified protein C,and S-2238.The results showed that after incubation with more than 25μg/ml LDL,activated protein C(APC)generation was significantly decreased,indicating that TM activity had been suppressed.There was a considerable negative correlation between LDL concentration and TM activity(r=-0.87,n=286, P< 0.001).The longer LDL incubation time,the more inhibition of TM activity achieved.The inhibition of six hours incubation was significantly more than that of two hours.Dynamic analysis showed that pre-incubation with LDL resulted in inhibition of whole process of APC generation,and decline of APC generation rate,especially in early period,so the radius of APC generation rate and reaction time curve was enlarged.The reaction rate of APC generation decreased in early stage but increased later.These data suggest that elevated blood level of LDL may contribute to decreasing the function of local anticoagulation by inhibiting the activity of TM on the vessel well.

关 键 词：低密度脂蛋白 内皮细胞 抗凝功能 动脉粥样硬化 

分 类 号：R543.5[医药卫生—心血管疾病] R329.26[医药卫生—内科学]