毛细管气相色谱法测定比阿培南中5种有机溶剂残留量  

Cotent determination of five residual organic solvents in biapenem by headspace capillary gas chromatography

作　　者：田晨煦[1] 徐小平[1] 廖丽云[1] 谭培[1] 任霞[1] 

机构地区：[1]四川大学华西药学院

出　　处：《中国抗生素杂志》2006年第3期187-189,共3页Chinese Journal of Antibiotics

摘　　要：目的建立比阿培南中甲醇、乙醇、二氯甲烷、乙腈和N,N-二甲基甲酰胺5种有机溶剂残留量的测定方法。方法采用毛细管气相色谱顶空进样法,氢火焰离子化检测器F(ID),以二甲基亚砜为溶剂,石英毛细管柱OV-170l(15m×0.53m m,1.0μm);柱温:50℃恒温6m in,以30℃/m in的速率升温至l80℃,恒温8m in;载气:高纯氮气2.0m lm/in;氢气:40m 1m/in;空气:300m l/m in。结果方法精密度为0.795%～2.25%,平均回收率99.2%～101.9%R(SD≤2.8%)。3批样品中5种有机溶剂残留量均符合要求。结论本法简便灵敏、准确、重复性好,能较好地进行比阿培南中有机溶剂残留量的测定。Objective To determine the contents of five residual organic solvents： methanol, ethanol, dichlorom ethane, acetonitrile, N, N-dimethylformamide in biapenem. Methods Samples dissolved in dimethyl sulfoxide was subjected to OV-17 capillary column （15m ×0.53mm, 1.0μm） with FID detector and programmed temperature. Nitrogen was used as the carrier gas： 2. 0ml/min, hydrogen： 40ml/min, air： 300ml/min. The initial temperature was kept at 50℃ for 6 min and increased at the rate of 30℃/min,up to 150℃for 8min. Results The precision were 0. 795% ～ 2.25%, the average recovery were 99.2% ～ 101.9% （RSD ≤2.8% ）. Five residual organic solvents in three batches of samples coincided with the requirements. Conclusion The method was proved to be accurate, sensitive and suitable to the determination of residual organic solvents in biapenem.

关 键 词：毛细管气相色谱法 比阿培南 有机溶剂残留量 

分 类 号：R927.1[医药卫生—药学]