矽尘染毒大鼠体内脂质过氧化与抗氧化的水平  

Levels of Lipid Peroxidation and Antioxidation in Silica Exposed Rats

作　　者：张中兴 吴开国[2] 杨莉[2] 袁秀玲[2] 

机构地区：[1]广西壮族自治区卫生监督所,广西南宁530021 [2]广西医科大学公共卫生学院,广西南宁530021

出　　处：《工业卫生与职业病》2006年第2期74-75,共2页Industrial Health and Occupational Diseases

摘　　要：目的探讨脂质过氧化与矽肺形成的关系。方法采用非暴露气管注入染尘法复制大鼠矽肺模型,检测SOD,GSH-Px,MDA,LDH和BALF等指标,同时做肺组织病理切片。结果染尘组大鼠红细胞SOD活力和全血GSH-Px活力与对照组比较,差异均有显著性(P<0.05);BALF中ACP、LDH的含量和肺组织中MDA的含量与对照组比较,差异有非常显著性(P<0.01),染尘组大鼠肺组织病理切片可见Ⅱ级和Ⅲ级细胞纤维性矽结节病变存在,而对照组未发现有上述病理改变。结论SiO2粉尘诱发了肺组织的脂质过氧化反应,促进了矽肺的形成。Objective To study the levels of lipid peroxidation and antioxidation in silica exposed rats. Methods Rats were divided randomly into control group and silica exposed group; silicosis model was prepared by intratracheal instillation. SOD, GSH-Px, MDA, LDH and BALF were tested simultaneously with pathological examination of lung tissues. Results The activities of SOD and GSH-Px in silica exposed group were obviously lower than those of control group （P〈0.05）, but the contents of ACP, LDH and MDA were measurably higher （P〈0.01）. There were Ⅱ degree and Ⅲ degree pathological silica nodules of cell fibrosis in exposed group, but nothing was found in control group. Conclusions The level of lipid peroxidadion in silica exposed rats was remarkably enhanced, but the level of antioxidation was notably lowered.

关 键 词：矽尘 大鼠 气管注入 脂质过氧化 抗氧化 

分 类 号：R135.2[医药卫生—劳动卫生]