RNA干扰技术的应用研究进展  

The Applications of RNA Interference Technique

作　　者：信吉阁[1] 王晓洪 韩佃刚[1] 牛自兵[1] 李月涛[1] 曾养志[1] 

机构地区：[1]云南农业大学动物科学技术学院,云南昆明650201 [2]辽宁抚顺市矿务局总医院,辽宁抚顺113008

出　　处：《动物医学进展》2006年第2期30-33,共4页Progress In Veterinary Medicine

摘　　要：RNA干扰是指由双链RNA(double-stranded RNA,dsRNA)启动的序列特异的转录后基因沉默现象,广泛存在于真菌、植物和动物中。RNA干扰技术是近年来迅速发展起来的高效、特异、易操作的基因阻断技术,在功能基因组的研究中有着广阔的应用前景。文章对RNA干扰的分子机制、生物学功能和RNA扰技术的优势以及其在功能基因组、基因治疗、转基因动物研究、药物开发等方面的应用做一综述。RNA interference （RNAi） is the process of sequence-specific, post-transcriptional gene silencing initiated by double-stranded RNA, which widely exists in epiphyte, plants and animals. RNAi technology is a newly developed simple gene-silence tool with high specificity and high efficiency being applied in functional genome. The article reviewed the molecular mechanism and biological function of RNAi and the predominance of RNAi technique as well as its applications in functional genome, gene therapy, transgenic animals, medicine development, etc.

关 键 词：RAN干扰 RNA干扰技术 功能基因组 基因治疗 

分 类 号：Q522[生物学—生物化学] Q752