从克隆的cDNA拯救狂犬病毒HEP-Flury株  

Rescue of HEP-Flury Strain of Rabies virus from Cloned cDNA

作　　者：艾军[1] 林宝珍[1] 郭霄峰[1] 

机构地区：[1]华南农业大学兽医学院,广东广州510642

出　　处：《动物医学进展》2006年第2期99-100,共2页Progress In Veterinary Medicine

基　　金：广东省自然科学基金(5200638)

摘　　要：为研究狂犬病毒基因的结构,将狂犬病毒HEP-Flury株全基因组cDNA与N、P、L、G 4个基因的辅助质粒共转染BHK-21细胞,于体积分数为5%CO2培养箱中培养6d,以狂犬病毒N蛋白荧光抗体染色。结果显示,细胞中含有大量的狂犬病毒粒子,表明已建立了狂犬病毒的反向遗传操作系统。To study the genes of Rabies virus, the full-length genome cDNA of the HEP-Flury strain and four helper plasmids with N, P, L and G gene respectively were co-transfected to BHK-21 cell. After incubation in 5% CO2, the cells were stained with fluorescence antibody of anti-N protein MAb. The results showed that the rabies virus was efficiently recovered from the clone eDNA and Rabies Virus reverse genetic system was developed.

关 键 词：狂犬病毒 反向遗传操作技术 HEP-Flury株 

分 类 号：S852.659.1[农业科学—基础兽医学]