甘露糖结合凝集素基因多态性与肺结核易感性的研究  

Sequence variations in the mannose binding lectin gene and their possible relationship in susceptibility to tuberculosis in the Chinese Han Nationality

作　　者：刘玮[1] 张芳[1] 赵秋敏[1] 吴晓明[1] 张翠英[2] 田磊[3] 李春芝 张泮河[1] 杨红[1] 曹务春[1] 

机构地区：[1]军事医学科学院微生物流行病研究所病原微生物生物安全国家重点实验室,北京100071 [2]解放军第309医院,北京100091 [3]解放军第91医院,山东兖州272100 [4]解放军总参三部防疫队,北京100091

出　　处：《中国热带医学》2006年第3期387-389,共3页China Tropical Medicine

基　　金：国家自然科学基金(30271144)

摘　　要：目的探讨甘露糖结合凝集素(MBL)基因多态性与中国汉族人群肺结核发病间的关联。方法采用以医院为基础的病例对照研究设计,对结核病相关危险因素进行问卷调查。联合应用引物序列特异性PCR(PCR-SSP)和序列特异性寡核苷酸探针杂交(PCR-SSOP)等方法对MBL基因H／L、X／Y、P／Q和A／B四个多态性位点进行基因型分析及单倍体型分析。将各位点的基因型或单倍体型在病例组和对照组的分布进行比较,并将有显著作用的环境危险因素纳入进行多因素非条件logistic回归分析。结果 133名肺结核病例和177名对照纳入本研究,多因素分析调整结核病暴露史和疫苗接种史两个因素后,MBL基因的HL和LL基因型与疾病的保护作用显著相关,调整OR值(95％CI)分别为0．54(0．30-0．95)和0．50(0．26-0．96)。MBL基因XB组单倍体型与中国汉族人群肺结核发生的易感性显著相关,调整OR值(95％CI)为1．60(1．07-2．41)。而其余多态性位点,包括X／Y、P／Q和A／B位点与肺结核的发生均无显著性关联。结论中国汉族人群MBL基因多态性可能与汉族人群肺结核发生的易感性显著相关。Objective To investigate the association between genetic polymorphisms of marmose binding lectin （MBL） gcnc and pulmonary tuberculosis （P. tb） infection in Chinese Han Nationality. Methods A case control study was performed. The genotype and haplotype of four MBL single nucleotide polymorphisms （A/B, H/L, Y/X and P/Q） were determined using sequence specific priming- polymerase chain reaction （PCR- SSP） technique and PCR- sequence specific oligonueleotide probe （PCR- SSOP） assay. The genotypc and haplotype frequencies were compared between P. tb cases anA controls using an unconditional logistic regression model. Results 133 patients and 177 healthy subjects were recruited. Multifactoral logistic analysis disclosed MBL- HH and LL genotypcs were significantly associated with the protection from the disease, with the adjusted OR （95% CI） to be 0.54（0.30 - 0. 95） and 0.50（0.26 - 0.96）, respectively. The XB haplotype group was present at a significantly higher frequency in the patients group than those with the YA group, with OR （95% CI） of 1.60（ 1.07 - 2.41）. Conclusion The sequence variations in the MBL gene might be associated with the susceptibility to P. tb in the Chinese Han Nationality.

关 键 词：肺结核 甘露糖结合凝集素基因 多态性 病例对照研究 

分 类 号：R521[医药卫生—内科学]