人巨噬细胞源性泡沫细胞分化中MaxiK通道α-亚单位的表达(英文)  

The Expression of MaxiK Channel α-subunit during Human Macrophages Differentiating into Foam Cells

作　　者：雷新军[1] 马爱群[1] 席雨涛[1] 张葳[1] 姚艳[1] 杜媛[1] 王亭忠[1] 耿涛[1] 

机构地区：[1]西安交通大学医学院第一附属医院心内科离子通道病研究室环境与疾病相关基因教育部重点实验室,西安7100611

出　　处：《四川大学学报（医学版）》2006年第2期171-175,共5页Journal of Sichuan University(Medical Sciences)

基　　金：纽约中华医学基金会基金(CMB,01-761);科技部国际合作重点项目(2003DF000037)资助~~

摘　　要：目的研究人单核细胞源性巨噬细胞向泡沫细胞分化过程中MaxiK通道α-亚单位的表达。方法采用密度梯度离心法从男性健康志愿者外周血中分离单核细胞,经培养后分化为巨噬细胞,并通过加氧化型低密度脂蛋白(OxLDL),建立人巨噬细胞源性泡沫细胞模型,采用RT-PCR、蛋白质印迹及免疫细胞化学方法研究MaxiK通道α-亚单位的表达。结果巨噬细胞同30mg/LOxLDL孵育60h后,细胞内总胆固醇(TC),游离胆固醇(FC)及胆固醇酯(CE)显著增加,并且CE/TC从(14.437±6.781)%提高到(57.946±3.507)%。同时,MaxiK通道α-亚单位表达被下调,但同巨噬细胞相比差异无统计学意义(P>0.05)。结论人单核细胞源性巨噬细胞同30mg/LOxLDL孵育60h后可分化为泡沫细胞,但MaxiK通道α-亚单位的表达无明显改变。Objective To investigate the expression of MaxiK channel α-subunit during human monocyte-derived macrophages differentiating into foam cells. Methods Human peripheral blood monocytes were isolated from male healthy volunteers by density gradient centrifugation, which, by culture, differentiated further into macrophages as a homogeneous monocyte population. The foam cell model originated from human macrophage was established by incubating macrophages with oxidized low density lipoprotein （OxLDL）. The expression of MaxiK channel α-subunit was investigated by RT-PCR techniques, Western blotting and immunocytochemistry. Results After incubating macrophages with 30 mg/L OxLDL for 60 hours, the cellular contents of total cholesterol （TC）, free cholesterol （FC） and cholesterol ester （CE） were markedly increased and the ratio of CE/TC was further raised from （14.437 ± 6.781）% to （57.946 ± 3.507） %. Although the expression of MaxiK channel α-subunit was downregulated during human monocyte-derived macrophages differentiating into foam cells, there was no significant difference between macrophages and foam cells （ P 〉 0.05）. Conclusion That 30 mg/L OxLDL can lead the monocyte-derived macrophage cultured for 60 hours to differentiate into foam cell, but the expression of MaxiK channel α-subunit does not change obviously.

关 键 词：离子通道 胆固醇代谢 细胞分化 动脉粥样硬化 

分 类 号：R392[医药卫生—免疫学]