T4、φχ174D和f2三种噬菌体对戊二醛消毒剂抵抗力的比较研究  

A Comparative Study on the Resistance of Bacteriophage T4, φχ174D, and f2 to Glutaraldehyde in Laboratory

作　　者：陈昭斌[1] 张朝武[1] 许欣[1] 朱敏[1] 王国庆[1] 

机构地区：[1]四川大学华西公共卫生学院医学检验学教研室

出　　处：《四川大学学报（医学版）》2006年第2期270-273,共4页Journal of Sichuan University(Medical Sciences)

基　　金：深圳市科技和信息局立项项目(深科信[2005]227号;项目序号244)资助

摘　　要：目的筛选出消毒实验中评价消毒因子对病毒灭活效果的指示噬菌体。方法比较研究噬菌体T4、φχ174D和f2对戊二醛消毒剂的抵抗力。消毒剂对噬菌体的灭活效果采用悬液定量灭活试验、中和剂鉴定试验、噬菌体的检测采用双层琼脂法。结果13000mg/L的戊二醛对噬菌体T4作用20min,或6000mg/L的戊二醛对噬菌体T4作用5min即可达到消毒水平(对噬菌体T4的灭活对数值(LIV)或噬菌体T4的对数减少值〔LRV)(log10No-log10Nt)≥4.00log10〕;22500mg/L的戊二醛对噬菌体φχ174D作用20min,或5000mg/L的戊二醛对噬菌体φχ174D作用5min达到消毒水平;34000mg/L的戊二醛对噬菌体f2作用40min,或8000mg/L的戊二醛对噬菌体f2作用10min达到消毒水平。结论上述三种噬菌体对戊二醛的抵抗力由强到弱为:噬菌体f2>噬菌体T4>噬菌体φχ174D。Objective To identify the best indicative bacteriophage in disinfection tests through comparing the resistance of bacteriophage T4,ψX174D, and f2 to glutaraldehyde. Methods The virucidal activities of glutaraldehyde against bacteriophage T4, ψX174D, and f2 were assessed with suspension tests along with neutralizer tests. The double-agar-layer plaque technique was used to detect the bacteriophage T4, ψX174D, and f2. Results （1）In a condition of 3000 mg/L of glutaraldehyde and 20 minutes of contact or 6000 mg/L of glutaraldehyde and 5 minutes of contact, ＂disinfection＂ level for bacteriophage T4 was achieved, with the log^0 inactivation value （LIV） or log10 reduction value （LRV） （=log10No-log10Nt）≥4.00 log10. （2） In a condition of 2500 mg/L of glutaraldehyde and 20 minutes ：of contact or 5000 mg/L of glutaraldehyde and 5 minutes of contact, the LIV for bacteriophage ψX174D reached ＂disinfection＂ level; （3） In a condition of 4000 mg/L of glutaraldehyde and 40 minutes of contact or 8000 mg/L of glutaraldehyde and 10 minutes of contact, the LIV for bacteriophage f2 reached ＂disinfection＂ level. Conclusion Bacteriophage f2 and bacteriophage ψX174D has the strongest and weakest resistance to glutaraldehyde respectively.

关 键 词：噬菌体T4 噬菌体ψX174D 噬菌体F2 戊二醛 抵抗力 

分 类 号：R187[医药卫生—流行病学]