抗AIB1-N单克隆抗体的制备及鉴定  

Production and Identification of Monoclonal Antibody Against N Terminal of AIB1

作　　者：庞秋霞[1] 彭晓东[2] 郄明容[3] 朱月明[4] 彭旭红[5] 董薇[5] 张林[5] 屈艺[6] 

机构地区：[1]四川大学华西基础医学与法医学院生物化学与分子生物学实验室,成都610041 [2]四川大学华西医院临床免疫室 [3]四川大学生命科学学院 [4]四川大学华西第二医院妇产科 [5]四川大学基础医学与法医学院免疫实验室 [6]四川大学华西第二医院实验中心

出　　处：《四川大学学报（医学版）》2006年第2期278-280,297,共4页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金创新研究群体科学基金(批准号30221001);四川省青年基金(编号04ZQ026-027及04SG022-009-2)资助

摘　　要：目的获得有生物活性的抗乳腺癌扩增性抗原1氮端(amplifiedinbrestcancer1-Nterminal,AIB1-N)单克隆抗体。方法以谷胱甘肽转硫酶耦联的抗乳腺癌扩增性抗原1氮端蛋白(GST-AIB1-N)为免疫原,免疫BALB/c小鼠,采用杂交瘤技术制备抗AIB1-N单克隆抗体,用间接ELISA和Western-blot鉴定其亚类和抗原结合特异性。结果成功筛选出一株稳定分泌抗AIB1-N单克隆抗体的杂交瘤细胞株,其分泌抗体亚类为IgG1,经Western-blot鉴定该McAb特异性高,亲和力强。结论成功制备了抗AIB1-N单克隆抗体,为深入研究AIB1的表达及临床应用提供了有力的工具。Objective To make and identify the monoclonal antibody against AIB1-N. Methods BALB/e mice were immunized with purified GST-AIB1-N protein, MeAb against AIB1-N was produced by hybridoma technique. ELISA and Western-blot were used to identify the immunoglobin subtype and specificity. Results A hybridoma cell was successfully produced to secrete the MeAb against AIB1-N, which was identified to belong in IgG1 subtype. By western-blot, the MeAb against AIB1 displayed strongly specificity and high affinity. Conclusion The MeAb against AIB1 protein may be a useful tool for studying the biological properties of AIB1 expression and the clinical laboratory detection.

关 键 词：AIB1-N蛋白 单克隆抗体 制备和鉴定 

分 类 号：R392[医药卫生—免疫学]